Systematic Approach To Engineer Escherichia coli Pathways for Co-utilization of a Glucose?Xylose Mixture

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Title:	Systematic Approach To Engineer Escherichia coli Pathways for Co-utilization of a Glucose?Xylose Mixture
Authors:	Chung-Jen Chiang;Hong Min Lee;Hong Jhih Guo;Zei Wen Wang;Li-Jen Lin;Yun-Peng Chao
Contributors:	保健營養生技學系
Keywords:	mixed sugars;metabolic engineering;biofuel;substituted chemicals
Date:	201310
Issue Date:	2013-11-01 02:15:49 (UTC+0)
Abstract:	Glucose and xylose are two major sugars of lignocellulosic hydrolysate. The regulatory program of catabolite repression in Escherichia coli dictates the preferred utilization of glucose over xylose, which handicaps the development of the lignocellulose-based fermentation process. To co-utilize a glucose–xylose mixture, the E. coli strain was manipulated by pathway engineering in a systematic way. The approach included (1) blocking catabolite repression, (2) enhancing glucose transport, (3) increasing the activity of the pentose phosphate pathway, and (4) eliminating undesirable pathways. Moreover, the ethanol synthetic pathway from Zymomonas mobilis was introduced into the engineered strain. As a consequence, the resulting strain was able to simultaneously metabolize glucose and xylose and consume all sugars (30 g/L each) in 16 h, leading to 97% of the theoretical ethanol yield. Overall, this indicates that this approach is effective and straightforward to engineer E. coli for the desired trait.
Relation:	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 61(31):7583–7590.
Appears in Collections:	[Department of Food Nutrition and Healthy Biotechnology] Journal Article

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