Abstract: Recently, there is an increasing interest on bicycle riding for recreation or fitness purpose. Bicycles are also accepted as urban transportation due to the consciousness of environmental protection. For a more comfortable riding experience, many bicycles are equipped with a suspension system including a front suspension fork and/or rear suspension. However, when a suspension system is added to a bicycle, it makes riding a little heavier since suspension dissipates some pedalling energy. This paper discusses front and rear suspensions corresponding to rider comfort and pedalling effort when riding on a rough road and smooth road. A human body computer model LifeMOD® is employed to model the cyclist. Dynamic analysis software ADAMS® is employed to analyze human body vibration and leg muscle forces of bicycle riding. Human body acceleration vs. vibration frequencies are used as the comfort criteria. The results show that a suspension system may effectively reduce high frequency vibration of the human body when riding on a rough road. Pedalling forces are mostly contributed by the biceps femoris and semitendinosus. The suspension system would increase the pedaling forces of femoris and semitendinosus. Other leg muscles have a minor effect on pedaling forces. Results obtained from this research are useful for the design of bicycle suspension systems with better comfort and less loss of pedalling efficiency.