The proposed method uses an arbitrary splitting plane to cut multiple large volume datasets in a 3D volume visualization environment. The splitting plane divides each volume dataset into two parts and each part can be assigned with a different transfer function. Because these volume datasets are too large to be put into the graphics memory, the multi-resolution method is also applied into this system. herefore, the construction of splitting plane has to deal with the issues of multiple datasets and level-of-detail (LOD). Moreover, we also provide a two-pass rendering solution to keep the correct rendering order with partial performance decreasing, if users want to draw color or map texture on the splitting plane. This functionality enables us to clearly explore more features inside these volume datasets.