The measurement of apolipoprotein A-I in human plasma by electroimmunoassay

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Title:	The measurement of apolipoprotein A-I in human plasma by electroimmunoassay
Authors:	Miller JP;Mao SJT;Patsch JR;Gotto AM Jr
Contributors:	Department of Biotechnology
Date:	1980-08
Issue Date:	2009-11-26 01:43:41 (UTC+0)
Publisher:	Asia University
Abstract:	A variable proportion of the total apolipoprotein A-I (apo A-I) present in plasma or high density lipoproteins (HDL) is normally detectable by immunochemical methods. This has been attributed to masking of some of the immunoreactive sites of apo A-I by lipid in the intact HDL particle. This difficulty has been circumvented by heating or delipidation. We find that exposure of plasma to concentrations of urea greater than about 7.0 M in the barbital buffer used to dilute plasma samples for estimation by electroimmunoassay enables the complete detection of Apo A-I, as judged by comparison with samples delipidated with tetramethylurea. The need for time-consuming heating or delipidation is avoided.
Relation:	Journal of Lipid Research 21(6):775-80
Appears in Collections:	[Department of Biotechnology] Journal Article

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