Quantitative immunoassay of total cellular GAP junction protein connexin32 during liver regeneration using antibodies specific to the COOH-terminus

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题名:	Quantitative immunoassay of total cellular GAP junction protein connexin32 during liver regeneration using antibodies specific to the COOH-terminus
作者:	Hendrix EM;Skowron‑Lomneth C;Wilfinger WW;Hertzberg EL;Mao SJT;Chen L Larsen WJ
贡献者:	Department of Biotechnology
日期:	1992
上传时间:	2009-11-26 01:43:24 (UTC+0)
出版者:	Asia University
摘要:	A radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) were used to determine relative concentrations of liver connexin32 (CX32) in rats. The RIA and ELISA utilize synthetic peptides corresponding to regions of the carboxyl-terminus and antibodies raised in rabbits against these peptides. Assuming that affinities of antisera are similar for peptide and native CX32, total cellular CX32 was found to exceed the amount of gap junction protein at the cell surface calculated from morphometric analyses by 1.5-2.0 fold. This finding raises the possibility that some of the protein is present in cytoplasmic compartments or as occult precursors in the plasma membrane. Studies of CX32 content in regenerating rat liver support this conclusion and show a time course of loss and recovery of CX32 that agrees with those reported in studies using other techniques
關聯:	Tissue & Cell 24(1):61-73
显示于类别:	[生物科技學系] 期刊論文

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