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    题名: SSCP analysis in the D-loop region of porcine mitochondrial DNA as confirmed by sequence diversity
    作者: Lin CS;Liu CY;Wu HT;Sun YL;Chang LC;Yen NT;Yang PC;Huang MC and Mao SJT.
    贡献者: Department of Biotechnology
    关键词: Animal breeding;Breeding;Mitochondrial DNA;Single strand conformation polymorphism
    日期: 1998-03
    上传时间: 2009-11-26 01:43:18 (UTC+0)
    出版者: Asia University
    摘要: Sequence polymorphism of the displacement loop (D-loop) region in mitochondrial DNA (mtDNA) is a cytoplasmic DNA marker. Since the analysis of single-strand conformation polymorphism (SSCP) has been used for detection of substitutions in nucleotide sequence, this technique was employed to investigate the porcine sequence polymorphism in the D-loop region. The primers were prepared to amplify the most polymorphic site with 392-bp in the D-loop region from whole blood of pigs. The PCR-SSCP patterns were identical in European breeds Landrace and Duroc, but were different in Yorkshire and in Taiwanese breeds Lanyu and Taoyuan. Nucleotide sequence analysis in the 392-bp PCR products were completely in accordance with the PCR-SSCP patterns. There was only a single nucleotide substitution between Yorkshire and Landrace/Duroc pig and 2-3 nucleotide substitutions (dependent on the breed and individual) between Taiwanese Lanyu and Taoyuan. As many as 10-11 nucleotides were different between European and Taiwanese breeds in which most differences clustered within a certain region in the D-loop. The results suggest that PCR-SSCP analysis may be used for detecting single nucleotide substitution in the porcine mtDNA D-loop region and show the diversified sequence in this region between European and Asian origins.
    關聯: Journal of Animal Breeding and Genetics 115(1):73-78
    显示于类别:[生物科技學系] 期刊論文

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