Inhibition of neointimal formation in porcine coronary artery by a Ras mutant

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題名:	Inhibition of neointimal formation in porcine coronary artery by a Ras mutant
作者:	Wu CH;Lin CS;Hung JS;Wu CJ;Lo PH;Jin G;Shyy YJ;Mao SJT
貢獻者:	Department of Biotechnology
日期:	2001-07
上傳時間:	2009-11-26 01:43:16 (UTC+0)
出版者:	Asia University
摘要:	BACKGROUND: Therapeutic approaches to reduce the neointimal formation caused by balloon injury have been focused mainly on experimental models of restenosis in the rat carotid artery. However, restenosis in rat carotid artery may not replicate the coronary arterial responses to injury in larger animals and humans. METHODS: In this study, we used pig coronary arteries as an animal model to evaluate the preventive effects of a virus-mediated dominant negative mutant RasN17 on balloon injury-induced restenosis. The viral particles were delivered to the balloon-injured coronary arteries via a dispatch catheter to keep the virus in a confined arterial segment for 10 min to reach optimal transfection. Six weeks after balloon injury, the pigs were sacrificed and the left anterior descending arteries were isolated for histological analysis. RESULTS: Neointima formation was prominent in the group receiving balloon injury as compared with the uninjured controls. A remodeling process with migration of collagen was also found in the injured coronary arteries. The application of AdRasN17 led to a 56% decrease in neointima formation and a 75% increase in lumen size, as compared with the balloon-injured vessels treated with AdLacZ control. CONCLUSIONS: These results suggest that AdRasN17 is an effective therapeutic gene in preventing balloon injury-induced neointimal formation in pig coronary arteries. Copyright 2001 Academic Press
關聯:	The Journal of Surgical Research 99(1):100-6
顯示於類別:	[生物科技學系] 期刊論文

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