This study was to develop a research of Humble of Jesus E-learning System for Christian Children’s Sunday school by the use of Animation software (Macromedia Flash MX)、HTML editor（Macromedia Dreamweaver MX）、digital image editor(PhotoImpact 8.0)、 computer hardware equipment. The main purpose was to assist students for studying after class. The material could show the Animation、text and sound at the same time. It improves the disadvantages of traditional teaching methods. We use these materials for teaching could reach some advantages, as shown follow: (1)E-learning System Animation materials could arise the interesting of studying Children’s Sunday school. (2)E-learning System Animation materials could do as a complemental teaching and complemental materials after Sunday school. (3)E-learning System Animation materials could improve the problems of individual differences between students. The purpose of the study was to examine the effect of using Assistive Technology in Christian Children’s Sunday school students. A quasi-experimental design was used in this study. This study consisted of 48 Sunday school students in two classes from the church. They were administered in two groups. The Assistive Technology in Children’s Sunday school Instructional Program was implemented in the experimental group、 while the control group was implemented in the traditional way. The major findings of research were as follows: （1）The experimental group scored higher in the Sunday school Achievement Test than the control group did. （2）The experimental group scored higher after using E-learning System for Christian Children’s Sunday school.
