Due to heavy workload and must being in shifts work, clinical nurses expose in different occupational hazards easily. The purpose of this study is was to investigate the status of nurses in occupational hazards and relevance with rotating work shift. A structural questionnaire was distributed to 806 nurses of a central Taiwan medical center, and descriptive statistics, t-test, Chi-square and logistic regression were applied to analyze the data. The major finding of this study indicated that 85.4% of sampled nurses had experienced occupational hurts within one year; felt pressure during work, ergonomics injury and stabbed by needle or knife when cleaning after used were the top three on the list. In a whole, younger and junior nurse who ignore the on job training of occupational hazard prevention courses were vulnerable to occupational hurt. Compared with the evening-shift nurse and night-shift nurse, day-shift nurse and fixed-shift nurse experienced lower incidence rate of occupational injury. The findings of this study established the relationship between the influence of rotating shift work and occupational hazard to nurse; wish can provide useful data for making effective injury prevention policy.