ASIA unversity:Item 310904400/31
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    Title: 地耳草大量繁殖之研究
    Mass propagation of Hypericum japonicum Thunb.ex Murray
    Authors: 容玉英
    Contributors: 保健營養生技學系碩士班
    Date: 2009
    Issue Date: 2009-10-09 07:43:01 (UTC+0)
    Publisher: 亞洲大學
    Abstract: 地耳草(Hypericum japonicum Thunb.ex Murray)為金絲桃科(Guttiferae)、金絲桃屬(Hypericum)一年生草本植物,本品始載於植物名實圖考。地耳草具有抗菌消炎、抗瘧、預防傳染性肝炎、胃腸疾病和抑制腫瘤等作用。現代藥理研究具有治療急、慢性肝炎。

    本省因土地開發及利用而破壞其生育地,為開發省產地耳草之生藥資源並解決地耳草生態保育問題及產業發展,本研究將野生地耳草莖段培養於含有 2 mg/l BA、0.2 mg/l NAA之 MS基本鹽類培養基中,初誘導出大量不定芽,供本研究探討各種不同因素影響之用。實驗結果顯示地耳草莖段誘導芽體增殖以含有2 mg/l Kinetin之1/2MS培養基可獲得10.2個芽體。若改將芽體培養於含有2 mg/l Kinetin及 5% 蔗糖之1/4MS培養基中,可得26.1條不定根。

    利用組織培養方法培養地耳草,不僅可克服土地、氣候及環境因素變化的限制,並可藉由建立地耳草組織培養以達到快速繁殖技術之目的。
    Appears in Collections:[Department of Food Nutrition and Healthy Biotechnology] Theses & dissertations

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