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Please use this identifier to cite or link to this item:
http://asiair.asia.edu.tw/ir/handle/310904400/2913
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| Title: | Mass propagation of Hypericum japonicum Thunb.ex Murray |
| Authors: | Jung Yu Ying |
| Contributors: | Department of Health and Nutrition Biotechnology????? |
| Keywords: | Hypericum japonicum Thunb. ex Murray;tissue culture;Guttiferae |
| Date: | 2009 |
| Issue Date: | 2009-11-16 08:08:05 (UTC+0) |
| Publisher: | Asia University |
| Abstract: | Summary
Hypericum japonicum Thunb. ex Murray, commonly called as “Di-Er- Cao”in Taiwan , is a species of Hypericum , Guttiferae genera , it was first recorded in Chin- Wu-Ming-Shih-Tu-Kao, has been as a Chinese herbal medicine for the treatment of several bacterial diseases, infectious hepatitis, gastrointestinal disorder, internal hemorrhage and tumors.
The pharmaceutical industry is mainly dependent upon wild populations for supplies of the plant material. Due to large-scale and over-harvesting of wild material from forests, H. japonicum is rapidly disappearing. The purpose of this study is to establish a tissue culture technique for the mass propagation of H. japonicum. In the study we found that the most efficient shoot formation was the nodal stem explants, cultured on MS medium supplemented with 2 mg/l BA, 0.2 mg/l NAA, then transferred onto solid medium containing 1/2 MS basic salts with 2 mg/l Kinetin, 1.0% agar and 3% sucrose, forming 10.2 shoot per explants the bast medium for rooting was 1/4 MS basic salts with 2 mg/l Kinetin and 5% sucrose, gave the highest root number of rooting. forming 26.1 root per explants.
Using tissue culture method of raise H. japonicum, not only to overcome the land, climate and environmental factor change limit, and by the establishment of tissue culture to H. japonicum rapid propagation techniques in order to achieve the purpose. |
| Appears in Collections: | [食品營養與保健生技學系] 博碩士論文
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