| Abstract: | This study focuses on the nutrient and heavy metal contents of the domestic Agaricus blazei Murrill and the relationships amongst casing materials, irrigation water, temperature, humidity, and carbon dioxide during the cultivation stage. The harvested domestic A. blazei fruiting bodies were divided into two groups, and each group was dried by means of either heat or frozen treatment. Then, the analysis of the contents of water, ash, crude protein, crude fat, cure fiber, carbohydrate, and heavy metal of the fruiting body was conducted. This research further analyzed the effect on mycelia growth of the four A. blazei strains (L04, L05, L06 from China, and L07 from Japan) under different temperature and cultivation media. An analysis of biomass and heavy metal contents was also conducted on the four test strains cultivated with different casing materials including loam, red soil, peat, and a mixture of red soil and peat. The results from this analysis show the optimum temperatures for cultivation of A. blazei mycelia with potato dextrose agar were 24? for strains L05 and L07 and 28? for strains L04 and L06. The data further demonstrate the various nutrient levels being: Water, 6.91-7.95 % ; Ash, 7.838.34% ; Crude protein, 22.2O23.l7% ; Crude fat, 3.86-4.85% ; Cure fiber, 6.Ol7.l7% ; Carbohydrate, 56.9759.58% ; S, 50.4-424.3 mg/lOOg; P, 675.0-887.0 mg/l00g ; Na, 45.O2O6.4 mg/l00g ; K, 2858.53320.3 mg/l0g ; Ca, 26.O3O.0 mg/l0g ; Mg, 9l.2l0.2 mg/l00g ; Fe, 8.7-42.6 mg/l00g ; Mn, 0.6-0.7 mg/l00g ; Cu, 4.4-5.9 mg/l00g ; Zn, 9.4-42.0 mg/bog; B, 0.3-M.4 mg/bOg ; Al, 16.8-17.5 mg/l00g. The data also show the heavy metal content levels being: Cr, 0.54?X0.65 ppm ; Ni, 0.07-0.13 ppm; As, 0.73-2.52 ppm; Cd, 4.71-5.12 ppm ; Hg, 0.10-0.11 ppm; Pb, 0.80-4.24 ppm. Biomass productivity level is the highest with strain L07 cultivated in loam D at 24.lg/bag. The other cultivation pairs were strain L05 and loam D at 15.8g/bag, strain L06 and loam D at 12.8 g/bag, and fmafly stain L07 cultivated in a mixture of red soil and peat produces the least amount of biomass at 4 g/bag. This experiment shows loam D is ideal for the cultivation of A. blazei. Additionally, elements such as cultivation room condition, sawdust quality, and water and temperature all require careful attention during the cultivation stage. In short, the results and the data obtained through this study can provide the vital information needed for the building of a safe cultivation model for the commercialization of A. blazei in the future. |
