Due to the rapid progress in technology and computer popularization, the scientific data is more important in medical treatment. We developed a pulse diagnosis and information system of Chinese Medicine. Unifying a set of simple fast and methods, using DAQ Card to pick up the physiological signal, and using some signal processing skills, we then achieved the scientific effect for Chinese medicine. By detecting the pulses of Chun, Guan and Chy, the doctor can view the pulse in the screen. The system has the function of real-time displaying the pulses, ECG and Heart Rate Variability (HRV) analysis. In addition, the database can improve the clinical application. Then, the doctor can acquire and analyze the pulse signals more conveniently.