In the main time, operation and chemotherapy are the major treatment for cancer. However in both ways are both harmful for patients, because chemotherapy kills both normal cell and cancer cell. Therefore, at present, most research facilities are working on target treatment which only kills cancer cell. Hence, our reach is going to build a tool for researcher to use and hope can help them could find the cancer gene pathway in the shortest time for reach our goal: Target Treatment. This study is gong to use the pathway graph from KEGG which was build by Kyoto University. Our tool will provide upload function which allow user to upload microarray data files. The basic idea of our study is to use t-test and fisher exact text to find out the significance gene and to map to EC number in pathway graph. In our study, after calculation the significance gene is only a few matches to EC number and this is what we need to change in the future. Furthermore, our tool will update as quick as KEGG web site.