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http://asiair.asia.edu.tw/ir/handle/310904400/2604
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| Title: | Determination of preservative contents from market-available wet noodle products and wet rice products in Nantou Area by HPLC |
| Authors: | tseng yueh jan |
| Contributors: | Department of Bioinformatics |
| Keywords: | to Traditional Chinese Medicine (Jung-Nien Lai) (Table 2-2). |
| Date: | 2008 |
| Issue Date: | 2009-11-06 14:32:39 (UTC+0) |
| Publisher: | Asia University |
| Abstract: | The aim of this study was to ensure the safety of market-available wet noodle products and rice wet products by investigating whether these products were contaminated with food additive or preservative or not. An HPLC system was applied to detect the concentrations of preservatives including sorbic acid, benzoic acid and dehydroacetic acid. In 2007, 41 market-available wet noodle products and 39 wet rice products were randomly selected from May to November for this study.
Solid samples were filtered by 0.45 μm filter after distillation, and liquid samples were diluted with 50% methanol in water, then filtered by 0.45 μm filter before sample injection. The samples were analyzed for sorbic acid, benzoic acid and dehydroacetic acid for 15 min by HPLC. The HPLC system equipped with a Supelco C18 15 cm × 4.6 mm, 5μm column. The mobile phase was methanol：acetonitrile：5 mM citric acid (1: 2: 7). The samples were analyzed with flow rate of 1ml/min and were monitored at λ=230 nm to detect benzoic acid, sorbic acid and dehydroacetic acid. The linear range was from 5 ppm to 100 ppm with correlation coefficient (r )= 1. IDL was 0.090 ppm for benzoic acid, 0.090 ppm for sorbic acid and 0.090 ppm for dehydroacetic acid. MDL was 0.090 ppm for benzoic acid, 0.093 ppm for sorbic acid and 0.090 ppm for dehydroacetic acid. The average recovery of standard spike added to samples were 98.380% for sorbic acid, 95.222% for benzoic acid and 97.864% for dehydroacetic acid. Blank test was 0% after triplicate studies. The average RPD was 0.926% for sorbic acid, 0.996% for benzoic acid, and 2.251% for dehydroacetic acid. The average recovery of standard spike addition were 101.625% for sorbic acid, 101.107% for benzoic acid, and 101.948% dehydroacetic acid.
In conclusion, 21 products out of 80 products including 8 wet noodle samples products and 13 wet rice products were found over the allowable preservative limitations. Four samples were found with sorbic acid, 12 samples were found with benzoic acid, and 5 samples were found with dehydroacetic acid. The total failed rate was 26.25%. |
| Appears in Collections: | [生物資訊與醫學工程學系 ] 博碩士論文
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