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http://asiair.asia.edu.tw/ir/handle/310904400/2595
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| Title: | Application of Genetic Techniques on Species Identification of Coral Reef fish Lutjanidae in Taiwan |
| Authors: | huang k?ng lin |
| Contributors: | Department of Bioinformatics |
| Keywords: | Genetic Techniques |
| Date: | 2007 |
| Issue Date: | 2009-11-06 14:32:30 (UTC+0) |
| Publisher: | Asia University |
| Abstract: | Ciguatera is one of marine fish poisoning to eat tropical or subtropical coral reef fish, it is estimated to affect more than 25,000 persons per year globally. The distribution of ciguatera is confined to discrete regions of the Pacific Ocean, western Indian Ocean and Caribbean Sea. The ciguateric toxins are produced by the toxic dinoflagellate Gambierdiscus toxicus, and are accumulated firstly in herbivorous fish and subsequently in carnivorous fish through the food chain effect. While some people eat the toxic fish, it will cause food poisoning. The Genus Lutjanus fish is one of carnivorous coral reef fishes that distributes around the sea area of Taiwan, and it is also one of the commercially important fish species in Taiwan. A food poisoning incident has occurred in February 2006 due to intake of the Lutjanus–like fish meat purchased from Cianjhen fishing market in Kaohsiung. Two patients of 48 years old showed diarrhea, muscle weakness, vertigo and severe muscle pain. These two poisoning samples were provided by Kaohsiung Chang-Gung Memorial Hospital and Tainan City Health Bureau, respectively. The results showed that these two poisoning samples contained 2.45 MU/g and 5.36 MU/g of toxins in the viscera extracts, respectively, but no death of mice was found in the muscle extracts. The other samples were collected from different fish markets but did not show any ciguateric symptoms and death of mice. In order to identify the fish species of poisoning samples, gene sequencing and polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) were used. The primer pair L14735/H15149 was used and a DNA fragment of 475 bp from cytochrome b gene was obtained. After gene sequencing, the species of both causative fish fillets was identified as Lutjanus bohar. Furthermore, restriction enzyme Hae III was found to differentiate all tested fish recollected from fish markets. Both causative fish fillets showed 3 fragments ( 183 bp, 151 bp and 134 bp ), which were the same as those of L. bohar in PCR-RFLP pattern. Therefore, the species of causative fish fillets was identified as L. bohar. |
| Appears in Collections: | [生物資訊與醫學工程學系 ] 博碩士論文
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