Chemical characterization and in vivo anti-inflammatory activities of Actinidia callosa var. ephippioides via suppression of proinflammatory cytokines.

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Title:	Chemical characterization and in vivo anti-inflammatory activities of Actinidia callosa var. ephippioides via suppression of proinflammatory cytokines.
Authors:	Liu, Chia-Yu;Liu, Chia-Yu;Li, Ying-Chih;Lin, Ying-Chih;鄧正賢;Deng, Jeng-Shyan;Lee, Chao-Yin;Lee, Chao-Ying;Li, Ying-Chih;Lin, Ying-Chih;Huang, Guan-J;Huang, Guan-Jhong
Contributors:	保健營養生技學系
Date:	2013-01
Issue Date:	2013-07-11 06:02:24 (UTC+0)
Abstract:	Actinidia callosa var. ephippioides (ACE) has been widely used to treat antipyretic, antinociceptive, anti-inflammation, abdominal pain and fever in Taiwan. This study aims to determine the mechanism of anti-inflammatory activities of ethyl acetate fraction of ACE (EA-ACE) using a model of lambda-carrageenan (Carr)-induced paw edema in mouse model. In HPLC analysis, chemical characterization of EA-ACE was established. In order to investigate the anti-inflammatory mechanism of EA-ACE, we have detected the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) and the levels of malondialdehyde (MDA) in the paw edema. Serum NO, tumor necrosis factor alpha (TNF-alpha), and interleukin-1 beta (IL-1 beta) were evaluated. Chemical characterization from HPLC indicated that EA-ACE contains betulinic acid, ursolic acid and oleanolic acid. In the antiinflammatory test, EA-ACE decreased the paw edema after Carr administration, increased the activities of CAT, SOD, and GPx and decreased the MDA level in the edema paw at the 5th hr after Carr injection. EA-ACE affects the serum NO, TNF-alpha, and IL-1 beta levels at the 5th hr after Carr injection. EA-ACE decreased Carr-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions by Western blotting. Actinidia callosa var. ephippioides have the potential to provide a therapeutic approach to inflammation-associated disorders.
Relation:	AMERICAN JOURNAL OF CHINESE MEDICINE, 41(2):405-423.
Appears in Collections:	[Department of Food Nutrition and Healthy Biotechnology] Journal Article

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