A wireless physiological signal measurement system has been developed in which physiological signals can be transferred to a host server via a personal digital assistant (PDA) with wireless transmission using Bluetooth technology. The subject’s personal information is also included with a radio frequency identification (RFID) tag. Both DES and RSA algorithms have been used to encrypt all the measured data and the key of DES algorithm to preserve the measured signal data with high security. This system is especially useful in the care and nursing environment, with efficient and high-accuracy security data transmission.