Improved DNA detection by utilizing electrically neutral DNA probe in field-effect transistor measurements as evidenced by surface plasmon resonance imaging

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題名:	Improved DNA detection by utilizing electrically neutral DNA probe in field-effect transistor measurements as evidenced by surface plasmon resonance imaging
作者:	陳文逸;Chen, Wen-Yih;陳泓成;Che, Hon-Chen;楊裕雄;Yang, Yuh-Shyong;黃俊仁;Huang, Chun-Jen;Hardy, Wai-Ho;Chan, Hardy Wai-Hong;胡文品;Hu, Wen-Pin
貢獻者:	生物與醫學資訊學系
日期:	2013-03
上傳時間:	2013-07-11 05:58:51 (UTC+0)
摘要:	Intensive efforts have been focused on the development of ultrasensitive DNA biosensors capable of quantitative gene expression analysis. Various neutralized nucleic acids have been demonstrated as alternative and attractive probe for the design of a DNA chip. However, the mechanism of the improvements has not been clearly revealed. In this investigation, we used a newly developed neutral ethylated DNA (E-DNA), a DNA analog with the "RO-P-O" backbone (wherein R could be methyl, ethyl, aryl, or alkyl group) obtained from synthetic procedures, and a silicon nanowire (SiNW) field-effect transistor (FET) to evaluate the difference in DNA detection performance while using E-DNA and DNA as probes. It is demonstrated that using the E-DNA probe in the FET measurement could have a significantly enhanced effect upon the detection sensitivity. Surface plasmon resonance imaging (SPRi) was used to evidence the mechanism of the improved detection sensitivity. SPRi analysis showed the amounts of probe immobilization on the sensor surface and the hybridization efficiency were both enhanced with the use of E-DNA. Consequently, neutral ethylated DNA probe hold a great promise for DNA sensing, especially in the electrical-based sensor. (C) 2012 Elsevier B.V. All rights reserved.
關聯:	BIOSENSORS & BIOELECTRONICS
顯示於類別:	[生物資訊與醫學工程學系 ] 期刊論文

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