Extraction of crude polysaccharide extracts from  Agricus brasiliensis and its effect on cellular viability in human hepatoma cells

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題名:	Extraction of crude polysaccharide extracts from Agricus brasiliensis and its effect on cellular viability in human hepatoma cells
作者:	jia jun Lu
貢獻者:	Department of Biotechnology
關鍵詞:	Agricus brasiliensis;hepatoma
日期:	2009
上傳時間:	2009-11-06 06:11:30 (UTC+0)
出版者:	Asia University
摘要:	Agaricus brasiliensis is a native mushroom of Brazil, South America. It is considered as an culinary-medical and functional food, a rare specie of mushroom rich in nutritious substance and living organism which enhance the immune system, antitumor, antioxidant,antivirus,and antimutagenic, abilities. Lately, scientific researchers have found that the polysaccharide of this mushroom is effective for the control of tumor cells. For this research, the fresh fruiting body of A. brasiliensis was refrigerated, dried, and ground into powder. With various temperatures of hot water or various times of Phosphate buffer saline combined with ultrasonic oscillation, 5.68 mg/ml of crude polysaccharides were extracted from 10g powders of Agaricus brasiliensis fruiting body by 100℃ hot wather, and 8.08 mg/ml polysaccharides by 15 min ultrasonic oscillation. The result also revealed that the quantity of polysaccharide contained in the cap of mushroom was 8.37mg/ml, which was higher than the 7.63mg/ml of polysaccharide contained in the stalk. The effects of crude polysaccharide on human liver cancer cell（HepG2?Hep3B）and normal liver cell（Chang liver cell）were studied using LDH assay and MTT assay to analyze the viability of liver cells. And using ALT assay and AST assay were used to analyze the metabolism of the liver cell function activity. The result showed that the Chang liver cell or liver cancer cell(HepG2, Hep3B) cultured with 0.32 mg/ml crude polysaccharide for 48 hours could increase Chang liver cells growth and reduce to LDH activity.On the other hand, the liver cancer cells (Hep G2，Hep3B) growth was restrained, yet their LDH activity was increased: HepG2 to 40% and Hep3B to 45%. The observation of microscope showed that cells of HepG2 and Hep3B were lysed after treating with A. brasiliensis crude polysaccharides. Some of cells appeared to be shrunk, empty and became smaller. The best concentration of crude polysaccharide among four treatments was 0.32 mg/ml. After 48 hour incubation, the AST and ALT activities of normal Chang liver cell were increased 5% and 60% indiviedually, where the AST and ALT of HepG2 were decreased 38% and 45%, and those of Hep3B were decreased 35% and 55% respectively.
顯示於類別:	[生物科技學系] 博碩士論文

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