ASIA unversity:Item 310904400/2492
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 94286/110023 (86%)
造访人次 : 21696584      在线人数 : 818
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://asiair.asia.edu.tw/ir/handle/310904400/2492


    题名: Development of enzyme optical detection system for the rapid detection of Escherichia coli
    作者: chang ts?n i
    贡献者: Department of Biotechnology
    关键词: E. coli;β-glucuronidase;4-methylumbelliferyl-β-glucuronide
    日期: 2007
    上传时间: 2009-11-06 06:10:40 (UTC+0)
    出版者: Asia University
    摘要: The aim of this study was to investigate whether the feasibility ofenzyme optical detection system could rapidly examine the Escherichia coli (E. coli) in food. The prototyping detection platform was built by integration of silicon photodiode, optic selective filter, photocurrent amplifier and digitize device. We make use of the β-glucuronidase (GUD) of particularity enzyme of E. coli, meeting will 4-methylumbelliferyl-β-glucuronide (MUG) resolve, producing 7-hydroxy-4- methylumbelliferyl outcome, and its outcome make use of the wave-length 366 nm to stir up the blue fluorescence that will produce the wave-length 449 nm. The production of blue fluorescence in the enzyme optical detection system was induced by the reaction between GUD of the E. coli and MUG. We can know GUD existence that the enzyme optical detection system detects the wave-length 449 nm the fluorescence, by this existence which examines E. coli. Furthermore, we used of Eosin Methylene Blue Agar (EMB) media and the sandwich ELISA method again to authenticate the E. coli that we separate.
    Detection of E. coli in the diet meat results in both enzyme optical detection system and traditional methods. However, detection with enzyme optical detection system takes only 12 hours while the traditional method needs 5 to 7 days. It’s no doubt that the enzyme optical detection system is provided with advantage of the efficiency and automation then that can be a traditional standard method used in detecting E. coli in food.
    Develop enzyme optical detection system to detect, can avoid an artificial subjective and last error margin and shorten time of examination, so fast and examine E. coli accurately, provide the food the safe and better guarantee.
    显示于类别:[生物科技學系] 博碩士論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    0KbUnknown477检视/开启
    2492.doc26KbMicrosoft Word167检视/开启


    在ASIAIR中所有的数据项都受到原著作权保护.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈