ASIA unversity:Item 310904400/1999
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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/1999


    Title: 馬兜鈴酸液相層析分析及中藥材毒性分類探討
    Survey of the Occurrence of Aristolochic Acid in Fangchi obtained from Mainland China by HPLC and The classification of the toxicity of traditional Chinese medicines
    Authors: 游尚航
    Contributors: 生物科技學系碩士班
    Date: 2009
    Issue Date: 2009-10-13 06:35:44 (UTC+0)
    Publisher: 亞洲大學
    Abstract: 本研究之目的在於調查大陸地區在禁用馬兜鈴酸藥材後的現狀調查。於2006年收集大陸各省份地區防己藥材共三十四家,以 4℃以下真空冷藏,並將樣品於前處理竭盡萃取完後入液相層析。
    分析方法流程為取 1.0 g 樣品粉碎加入 200 mL甲醇溶媒,超音波震盪 30 分鐘,濾紙過濾,濃縮定量至10 mL。高效液相層析儀分析馬兜鈴酸指標成分時,移動相為CH3CN / H2O / CH3COOH (45:53:2, v/v),流速為0.8mL/min,檢測螢光波長為 254 nm。利用上述分析技術進行對照標準品馬兜鈴酸分析,結果顯示六個樣品含有馬兜鈴酸。得線性迴歸方程式(Y=mX+b)及相關係數(r)均顯示良好線性關係。同日內相對標準偏差及回收率均在可接受範圍。
    Appears in Collections:[Department of Biotechnology] Theses & dissertations

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