黑點根腐病菌在台灣的寄主範圍

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题名:	黑點根腐病菌在台灣的寄主範圍
作者:	林益昇;Lin, Y.S
贡献者:	通識教育中心
关键词:	洋香瓜黑點根腐病,寄主範圍,Monosporascus cannonballus;root rot/vine decline of muskmelon,host range,Monosporascus cannonballus
日期:	2009-01
上传时间:	2012-11-26 04:12:15 (UTC+0)
摘要:	"Monosporascus cannonballus在田間侵害洋香瓜引起根腐，大部份罹病植株於採收前二星期產生急速萎凋(sudden wilt)的病微。在溫室內以土壞試管法、栽培盆接踵法與苗圃（350×132×24立方公分）接種法進行M. cannonballus的接種試驗，結果於土壞試管法中觀察到供試的10種瓜類作物，包括洋香瓜、香瓜、越瓜、胡瓜、冬瓜、南瓜、西瓜、蒲瓜、絲瓜與苦瓜等，其根圈附近，皆有子囊孢子發芽及菌絲浸入根表皮的情形。在栽培盆接種法中，洋香瓜接種株未出現與田間相同的萎凋病徴。採用苗圃接種法，則可完成柯電氏法則，確立病菌與病害的關係。田間調查得知洋香瓜黑點根腐病菌在台灣的寄主植物有：洋香瓜、香瓜、越瓜、胡瓜、蒲瓜（作西瓜根砧）和冬瓜等。除了洋香瓜發病嚴重外，其他作物黑點根腐病僅局限在彰化縣芳苑鄉小面積或溫室內發生。另外，本病原菌僅可由罹病根組織分離得到，自同一罹病株根腐指數為0-4各級的部位，所得病原菌分離率分別爲69、82、73、40與9％。 Monosporascus cannonballus caused root rot/vine decline of muskmelon. Sudden wilt of most infected plants occurred in the field at 2 weeks before harvesting. In greenhouse inoculation tests, the methods of soil tube inoculation, pot inoculation, and micro-plot (350×l32×24 cm^3) inoculation were employed. Using soil robe method, the ascospores could germinate in the rhizosphere and the hyphae penetrated into root tissues in such plants as muskmelon, Japanese cantaloupe, oriental pickling melon, cucumber, wax gourd, squash, watermelon, bottle gourd, loofah and bitter gourd. In pot inoculation, muskmelon plants did tot produce the wilting symptom hot they did in micro-plot inoculation tests, thus fulfilling the Koch's postulates. Field survey results showed that the host range of M. cannonballus included muskmelon, Japanese cantaloupe, oriental pickling melon, cucumber, wax gourd and bottle gourd as rootstock for watermelon in Taiwan. Besides the muskmelon, which was usually seriously damaged, the root rot/vine decline in other 5 cucurbit plants were limited to some locations in Fangyuan of Changhua County or in the greenhouse. In addition, this pathogen could only be isolated from infected roots. The isolation rates were low when the inoculum was taken from badly rot tissues of infected plants. Using toot tissues with a root rot index in the range of 0 to 4, the isolation rates obtained were 69, 82, 73, 40 and 9%, respectively."
關聯:	植物病理學會刊
显示于类别:	[通識教育中心] 期刊論文

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