Synergistic Apoptosis-Inducing Antileukemic Effects 4 of Arsenic Trioxide and Mucuna macrocarpa Stem 5 Extract in Human Leukemic Cells via a Reactive 6 Oxygen Species-Dependent Mechanism

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题名:	Synergistic Apoptosis-Inducing Antileukemic Effects 4 of Arsenic Trioxide and Mucuna macrocarpa Stem 5 Extract in Human Leukemic Cells via a Reactive 6 Oxygen Species-Dependent Mechanism
作者:	Kuan-Hung Lu;Hui-Ju Lee;Min-Li Huang;Shang-Chih Lai;Yu-Ling Ho;Yuan-Shiun Chang;Chin-Wen Chi
贡献者:	保健營養生技學系
日期:	2011
上传时间:	2012-11-26 02:32:45 (UTC+0)
摘要:	The objective of this study was to examine the potential of enhancing the antileukemic activity of arsenic trioxide (ATO) by combining it with a folk remedy, crude methanolic extract of Mucuna macrocarpa (CMEMM). Human leukemia cells HL-60, Jurkat, and Molt-3 were treated with various doses of ATO, CMEMM, and combinations thereof for 24 and 48 h. Results indicated that the combination of 2.5 μM ATO and 50 μg/mL CMEMM synergistically inhibited cell proliferation in HL-60 and Jurkat cell lines. Apoptosis triggered by ATO/CMEMM treatment was confirmed by accumulation of cells in the sub-G1 phase in cell cycle analyses, characteristic apoptotic nuclear fragmentation, and increased percentage of annexin V-positive apoptotic cells. Such combination treatments also led to elevation of reactive oxygen species (ROS). The antioxidants N-acetyl cysteine (NAC), butylated hydroxytoluene, and α-tocopherol prevented cells from ATO/CMEMM-induced apoptosis. The ATO/CMEMM-induced activation of caspase-3 and caspase-9 can be blocked by NAC. In summary, these results suggest that ATO/CMEMM combination treatment exerts synergistic apoptosis-inducing effects in human leukemic cells through a ROS-dependent mechanism and may provide a promising antileukemic approach in the future.
關聯:	Evidence-based Complementary and Alternative Medicine
显示于类别:	[食品營養與保健生技學系] 期刊論文

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