Curcumin-induced DNA damage and inhibited DNA repair genes expressions in mouse-rat hybrid retina ganglion cells (N18)

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 期刊論文 > Item 310904400/16340

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://asiair.asia.edu.tw/ir/handle/310904400/16340

题名:	Curcumin-induced DNA damage and inhibited DNA repair genes expressions in mouse-rat hybrid retina ganglion cells (N18)
作者:	呂旭峰;Lu, HF;楊家欣;Yang, JS;賴光啟;Lai, KC;徐素琴;Hsu, SC;Hsueh, SC;Chen, YL;江若華;Chiang, JH;呂啟誠;Lu, CC;羅琦;Lo, C;楊美都;Yang, MD;鍾景光;Chung, Jing-Gung
贡献者:	生物科技學系
关键词:	Curcumin;DNA damage;DNA repair;N18 mouse–rat hybrid retina ganglion cells
日期:	2009-08
上传时间:	2012-11-23 09:11:42 (UTC+0)
摘要:	Curcumin is reported to be a potent inhibitor of the initiation and promotion of many cancer cells. We investigated to examine whether or not curcumin induce DNA damage in mouse–rat hybrid retina ganglion cell line N18 cells. The Comet assay showed that incubation of N18 cells with 10, 25 and 30 μM of curcumin led to a longer DNA migration smear (Comet tail). The DNA gel electrophoresis showed that 20 μM of curcumin for 24 and 48 h treatment induced DNA damage and fragments in N18 cells. The real time PCR analysis showed that 20 μM of curcumin for 48 h treatment decreased ATM, ATR, BRCA1, 14-3-3σ, DNA-PK and MGMT mRNA, and ATM and MGMT mRNA expression were inhibited in a time-dependent manner. Our results indicate that curcumin caused DNA damage and inhibited DNA repair genes which may be the factors for curcumin-inhibited cell growth.
關聯:	NEUROCHEMICAL RESEARCH, 34(8):1491-1497.
显示于类别:	[Department of Biotechnology] Journal Article

文件中的档案:

没有与此文件相关的档案.

在ASIAIR中所有的数据项都受到原著作权保护.