Short-Term versus Long-Term Intermittent Hypobaric Hypoxia on Cardiac fibrosis and Fas-Death-Receptor Dependent Apoptotic Pathway in Rat Hearts

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Title:	Short-Term versus Long-Term Intermittent Hypobaric Hypoxia on Cardiac fibrosis and Fas-Death-Receptor Dependent Apoptotic Pathway in Rat Hearts
Authors:	;Lin, Yueh-Min;Huang, Shu-Kuei;Wang, Hsueh Fang;Chen, Li-Mien;Tsai, Fuu-Jen;Hsu, Hsi-Hsien;Kuo, Chia-Hua;Shyi-Gang, P.Wang;黃志揚;HUANG, CHIH-YANG;Lee, Shin-Da
Contributors:	生物科技學系
Keywords:	cardiac fibrosis;cell apoptosis;high altitude;Fas pathway;time course
Date:	2008-10
Issue Date:	2012-11-23 09:10:58 (UTC+0)
Abstract:	"It is unknown if short-term and long-term intermittent hypobaric hypoxic challenges both exert pro-apoptotic effects on Fas death receptor-dependent apoptotic pathway in rat hearts. Seventy-two Sprague-Dawley rats were randomly assigned into two groups. First, short-term intermittent hypobaric hypoxia (STIHH)-normobaric normoxia (n = 12), hypobaric hypoxia (380 mmHg, 12% O2 , 8 hrs/day) for 1 day (n = 12), and for 4 days (n = 12) and second, long-term intermittent hypobaric hypoxia (LTIHH)- normobaric normoxia (n = 12), hypobaric hypoxia for 1 week (n = 12) and 2 weeks (n = 12). After STIHH or LTIHH challenge, Fas receptor related pathway and histopathological analysis in the excised left ventricle was determined by Western blotting, RT-PCR, Hematoxylin-eosin staining, Masson trichrome staining and TUNEL assay. Fas death receptor and TNFα were significantly decreased after STIHH whereas Fas receptor, TNFα, FAS-associated death domain (FADD), and caspase 8 were increased after LTIHH. In addition, cardiomyocyte disarray and fibrosis were observed in 1 week LTIHH. Cardiac hypertrophy and more severe disarray, fibrosis and cardiac apoptotic activities were observed in 2 week LTIHH. STIHH exerts anti-apoptotic effects on hearts such as downregulation of TNFα and Fas receptor whereas LTIHH exerts pro-apoptotic effects such as upregulation of TNFα and Fas-mediated apoptotic pathways and lead to cardiac fibrosis and apoptosis. Our findings imply that short-term versus long-term intermittent hypobaric hypoxia exerted protective versus deleterious effects on hearts."
Relation:	CHINESE JOURNAL OF PHYSIOLOGY
Appears in Collections:	[Department of Biotechnology] Journal Article

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