HistoneQ1 Acetylation Is Essential for ANG-II-Induced IGF-IIR Gene  Expression in H9c2 Cardiomyoblast Cells and Pathologically Hypertensive Rat Heart

ASIAIR > College of Medical and Health Science > Department of Biotechnology > Journal Article > Item 310904400/16191

Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/16191

Title:	HistoneQ1 Acetylation Is Essential for ANG-II-Induced IGF-IIR Gene Expression in H9c2 Cardiomyoblast Cells and Pathologically Hypertensive Rat Heart
Authors:	黃志揚;HUANG, CHIH-YANG
Contributors:	生物科技學系
Date:	2011
Issue Date:	2012-11-23 09:09:43 (UTC+0)
Abstract:	The IGF-II/mannose 6-phosphate receptor (IGF-IIR/Man-6-P) up-regulation correlates with heart disease progression and its signaling cascades directly trigger pathological cardiac hypertrophy, fibrosis, and cardiomyocytes apoptosis. IGF-IIR gene expression/ suppression is able to prevent myocardial remodeling. However, the regulating mechanisms for the IGF-IIR gene remain unclear. This study performed reverse transcriptase PCR (RT-PCR) and methylation-specific PCR (MS-PCR) to detect expression and DNA methylation of CpG islands within the IGF-IIR genomic DNA region. Our finding revealed that the IGF-IIR gene was up-regulated both in H9c2 cells treated with tumor necrosis factor-alpha (TNF-α), lipopolysaccharide (LPS), angiotensin II (ANGII) and inomycin, and age-dependently in spontaneously hypertensive rat (SHR) heart. For the DNA methylation study, although there were four CpG islands within IGF-IIR genomic regions, the DNA methylation distribution showed no change either in cells treated with ANGII or in the SHR heart. Using chemical inhibitors to individually block histone acetyltransferase (HAT) and histone deacetylase (HDAC) activity, we found that histone acetylation was essential for ANGII-induced IGF-IIR gene expression using RT-PCR and luciferase assay. The Chromatin immuno-precipitation assay indicated that acetyl-Histone H3 and acetyl-Histone H4 associated with the IGF-IIR promoter increased in the presence of ANGII, otherwise methyl-CpG binding domain protein 2 (MeCP2) is disassociated with this. Taken together, this study demonstrates that histone acetylation plays a critical role in IGF-IIR up-regulation during pathological cardiac diseases and might provide a targeting gene in transcriptional therapies for the failing heart.
Relation:	JOURNAL OF CELLULAR PHYSIOLOGY, V.227 N.1:259-268.
Appears in Collections:	[Department of Biotechnology] Journal Article

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML	204	View/Open

Loading...