Rapid detection of two-protein interaction with a single fluorophore by using a microfuidic device.

ASIAIR > College of Medical and Health Science > Department of Biotechnology > Journal Article > Item 310904400/16167

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Title:	Rapid detection of two-protein interaction with a single fluorophore by using a microfuidic device.
Authors:	;Chou, CK;Jing, N;Yamaguchi, H;Tsou, PH;Lee, HH;Chen, CT;Wang, YN;Hong, S;Su, C;Kameoka, J;洪明奇;Hung, Mien-Chie
Contributors:	生物科技學系
Date:	2010
Issue Date:	2012-11-23 09:09:25 (UTC+0)
Abstract:	We have developed a microfluidics based platform and methodology named MAPS (microfluidic system for analyzing proteins in single complex) for detecting two protein interactions rapidly using a single fluorophore. Target proteins were labelled with Quantum dot 525 (QD525) via specific polyclonal antibodies, and were transported through the microfluidic channel subsequently, where the 375 nm excitation laser light was focused to form a detection volume. Photon bursts from target proteins passing through the detection volume were recorded and their photon burst histograms were plotted which demonstrated roughly the specific protein interaction ratio based on their population and statistical behavior. As a proof of concept, Src/STAT3 protein complex interaction ratios with and without EGF stimulation were obtained by MAPS within 1 h and the results were well matched with the one obtained by the conventional immunoprecipitation/Western blot (IP/WB).
Relation:	ANALYST
Appears in Collections:	[Department of Biotechnology] Journal Article

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