High speed digital protein interaction analysis using microfluidic single molecule detection system.

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 期刊論文 > Item 310904400/16158

請使用永久網址來引用或連結此文件: http://asiair.asia.edu.tw/ir/handle/310904400/16158

題名:	High speed digital protein interaction analysis using microfluidic single molecule detection system.
作者:	;Chou, CK.;Jing, N.;Yamaguchi, H;Tsou, PH;Lee, HH;Chen, CT.;Wang, YN.;Hong, S.;Su, C.;Kameoka, J;洪明奇;Hung, Mien-Chie
貢獻者:	生物科技學系
日期:	2010
上傳時間:	2012-11-23 09:09:18 (UTC+0)
摘要:	The understanding of protein interaction dynamics is important for signal transduction research but current available techniques prove difficult in addressing this issue. Thus, using the microfluidic approach, we developed a digital protein analytical platform and methodology named MAPS (Microfluidic system Analyzing Protein in Single complex) that can measure the amount of target proteins and protein complexes at the digitally single molecule resolution. By counting protein events individually, this system can provide rough protein interaction ratios which will be critical for understanding signal transduction dynamics. In addition, this system only requires less than an hour to characterize the target protein sample, which is much quicker than conventional approaches. As a proof of concept, we have determined the interaction ratios of oncogenic signaling protein complexes EGFR/Src and EGFR/STAT3 before and after EGF ligand stimulation. To the best of our knowledge, this is the first time that the interaction ratio between EGFR and its downstream proteins has been characterized. The information from MAPS will be critical for the study of protein signal transduction quantitation and dynamics.
關聯:	LAB ON A CHIP;10(14):1793-8.
顯示於類別:	[生物科技學系] 期刊論文

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
index.html		0Kb	HTML	407	檢視/開啟

在ASIAIR中所有的資料項目都受到原著作權保護.

資料載入中.....