Nestin serves as a prosurvival determinant that links to the cytoprotective effect of epidermal growth factor in rat vascular smooth muscle cells

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 期刊論文 > Item 310904400/16124

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題名:	Nestin serves as a prosurvival determinant that links to the cytoprotective effect of epidermal growth factor in rat vascular smooth muscle cells
作者:	黃元勵;Huang, Yuan-Li
貢獻者:	生物科技學系
關鍵詞:	nestin;apoptosis;EGF;caspase;CDK5;vascular smooth muscle cells
日期:	2009
上傳時間:	2012-11-23 09:08:51 (UTC+0)
摘要:	Nestin is an intermediate filament protein mainly expressed in muscle and neural progenitors. Recently, we reported that nestin is expressed in rat vascular smooth muscle cells (VSMCs), disappears after serum-deprivation and then is re-expressed again following EGF stimulation. As the function of nestin in VSMCs remains unknown, its anti-apoptotic function was investigated in this study. We first showed that cell viability of nestin-depleted cells following H(2)O(2) treatments decreased by nestin RNAi. Further DNA laddering analysis and flow cytometry results demonstrated that this loss of cell viability was mediated through apoptosis. In addition, caspase-9, caspase-3 and PARP were activated in nestin-depleted VSMCs following H(2)O(2) treatments, indicating that nestin has an upstream inhibitory effect on caspase activation. It is well known that EGF serves as a survival factor in rat VSMCs. Here, we show that the cytoprotective effect of EGF was prevented by nestin RNAi. In addition, the inhibition of Cdk5 prevented Bcl-2 phosphorylation and enhanced H(2)O(2)-induced caspase-3 activation as well as subsequent DNA fragmentation. Taken together, these results provide evidence for another cytoprotective role of EGF in that it is mediated through its stimulation of nestin expression which leads to the prevention of caspase activation by Cdk-5-induced Bcl-2 phosphorylation in rat VSMCs.
關聯:	JOURNAL OF BIOCHEMISTRY
顯示於類別:	[生物科技學系] 期刊論文

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