One-step immunochromatographic assay for the detection of Staphylococcus aureus.

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 期刊論文 > Item 310904400/16093

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题名:	One-step immunochromatographic assay for the detection of Staphylococcus aureus.
作者:	黃素華;Huang, Su-Hua
贡献者:	生物科技學系
关键词:	Immunochromatographic assay;Staphylococcus aureus;Anti-protein A IgG;Gold nanoparticles;Nitrocellulose membrane
日期:	2007
上传时间:	2012-11-23 09:08:27 (UTC+0)
摘要:	An analytical system of immunochromatographic assay based on gold nanoparticles was developed to detect Staphylococcus aureus. The assay was in the sandwich format, using anti-protein A IgG with two distinct specificities. One anti-protein A IgG was immobilized in a defined detection zone on a porous nitrocellulose membrane, while the other anti-protein A IgG was conjugated with gold nanoparticles. The mixture was then passed along the porous membrane by capillary action past the anti-protein A IgG in the detection zone, binding the particles to which surface protein A was already bound to their surface, yielding a red color. The sensitivity and specificity in the immunochromatographic test were 100 and 93.0–100% for 28 S. aureus strains and 23 non-S. aureus strains, respectively. S. aureus was detected by immunochromatographic testing of two samples of precooked foods naturally contaminated with the bacterium. Eleven processed foods artificially inoculated with S. aureus at <25 CFU/g, all yielded positive results in the immunochromatographic test.
關聯:	FOOD CONTROL,18(8),893–897.
显示于类别:	[生物科技學系] 期刊論文

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