This study was aimed at exploring not only the relations between work-family role values, role commitment, work hours input and work-family conflict, but also the mediating effect of work hours input during holidays between role commitment and work-family conflict. The purposive sampling method was used to conduct surveys with structured questionnaires among full-time workers in Taiwan. A total of 420 questionnaires were distributed and 410 participants returned valid responses for further analysis. The valid response rate was 97.6%. The results revealed that work role values had positive impacts on work role commitment, and family role values had positive impacts on family role commitment. Moreover, work role commitment was positively related to work hours input during holidays, however, family role commitment had negatively effect on work hours input. Finally, work hours input was positively related to work-family conflict and work hours input had partial mediating effects on the relations between role commitment and conflict in both work and family way.