ASIA unversity:Item 310904400/12434
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    题名: Investigation of Microarray Data Using Gene Set Enrichment Analysis - Arabidopsis thaliana infected with Xanthomonas campestris pv. campestris
    作者: Chen, Ching-yi
    贡献者: Department of Biomedical informatics
    Ka-Lok Ng
    关键词: Arabidopsis;Xanthomonas campestris pv. campestris （Xcc）;microarray;differentially expressed genes;gene set enrichment analysis
    日期: 2012
    上传时间: 2012-11-18 08:08:12 (UTC+0)
    出版者: Asia University
    摘要: Gaining a better understanding of the biotic and abiotic stress responses for plant systems provide a model system for studying human diseases and drug-related research. Understanding how plant systems defense against environment stress is of great significance for the world's food and agricultural production.In this study, the microarray data for Arabidopsis thaliana infected with Xanthomonas campestris pv. campestris （Xcc） is analyzed. Microarray data for Arabidopsis infected with Xcc are retrieved from the ArrayExpress database, where differentially expressed genes （DEGs） and Gene Set Enrichment Analysis (GSEA) for pathogen-resistant pathways are deduced by adopting the R language, Bioconductor and KEGG information. Finally, the found results are validated by published literature.It is found that the SGT1 and HSP90 protein complexes utilize the SKp1 protein in the ubiquitin - proteasome system to regulate the hypersensitivity resistance mechanism, which is mediated by the resistance protein RPM1.Furthermore, DEG results for Xcc under different experimental conditions, as well as bacteria Agrobacterium tumefaciens, are also determined. The mentioned results can be accessed at http://ppi.bioinfo.asia.edu.tw/R_At_xcc/index.htm .
    显示于类别:[生物資訊與醫學工程學系 ] 博碩士論文

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