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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/116892


    Title: 微量熱法評估不同光源照射側耳屬多醣對微生物的益生元影響
    Other Titles: The prebiotic effects on microorganisms growth by different lights irradiated Pleurotus spp. mushroom polysaccharides via microcalorimetry
    Authors: 蘇稀瑤
    SO, HEI-YIU
    Contributors: 林駿憑;蔡淑瑤
    LIN, CHUN-PING;TSAI, SHU-YAO
    食品營養與保健生技學系
    Keywords: 菇類;多醣;益生菌;植物乳桿菌;馬克斯克魯維酵母菌;微量熱法
    Mushroom;Polysaccharide;Probiotics;Lactobacillus plantarum,;Kluyeromyces marxianus;Microcalorimetry
    Date: 2023
    Issue Date: 2023-11-22 01:13:07 (UTC+0)
    Abstract: 食用菇類是日常飲食中不可缺少的成分，因為它們具有豐富的營養和對身體健康有益的特性。多醣是杏鮑菇和金頂側耳中最重要的活性成分之一，具有多種促進健康的功能。本研究選用植物乳桿菌（L. plantarum）和馬克斯克魯維酵母菌（K. marxianus）的益生菌菌株進行培養，以探討杏鮑菇多醣（PEP）和金頂側耳多醣（PCP）對其生長曲線的影響。除此以外，為了解不同光源照射下的菇類多醣對植物乳桿菌和馬克斯克魯維酵母菌生長的影響，我們把PEP照光（伽馬射線、脈衝光、發光二極體和冷陰極螢光燈照射），另外也把PCP照光（伽馬和脈衝光照射）。PEP和PCP的熱特性是通過微差掃描量熱卡儀（DSC）來測定。 研究結果顯示，與未受光源照射的多醣相比，一半以上被光照射的菇類多醣的起始溫度都輕微下降。在水分脫附過程中，當升溫速率為10 °C/min時，被伽馬射線照射的PEP的起始溫度都降低了。當中3-10、5-19 和9-30 kGy的起始溫度分別為 127.70、128.97和129.48 °C。而未經光源照射的PEP的起始溫度為 130.35 °C。相信被光源照射的菇類多醣結構被略為改變。光源照射可能導致多醣的結構變得較為鬆散，光線可能破壞了它們的分子結構。然而，在這項研究中觀察到的變化並不顯著。在等溫量熱卡儀（TAM Air）實驗中，未經光源照射和有受光源照射的菇類多醣都適合成為植物乳桿菌培養基中的碳源，所有植物乳桿菌的生長曲線皆令人滿意，由此可見植物乳桿菌可以消耗由PEP和PCP所提供的能量。另外，從結果可以觀察到，在混合含有菇類多醣和葡萄糖的培養基中，植物乳桿菌的生長曲線最佳。有趣的是，在對照組中植物乳桿菌的生長曲線，含有1%葡萄糖的培養基反而比含有2%葡萄糖的培養基表現更為理想，這表示過高的糖份濃度可能會阻礙植物乳桿菌的生長。此外，植物乳桿菌在僅含有菇類多醣的培養基中生長速度相對較低。分析中顯示PEP和PCP都不適合成為K. marxianus培養基中的碳源。 添加菇類多醣的培養基並沒有改善K. marxianus的生長結果。總結上述，PEP和PCP均適合部分或全部替代植物乳桿菌培養基中的碳源。另外，PEP和PCP不適合替代K. marxianus培養基中的碳源。 由此可見K. marxianus對碳源的選擇較為敏感，培養基中葡萄糖的濃度亦會直接影響K. marxianus的生長。
    Edible mushrooms are an essential ingredient of our daily diet since their abundant nutrition and valuable properties for body health. Polysaccharides from Pleurotus eryngii (PEP) and Pleurotus citrinopileatus (PCP), one of most important bioactive components in mushrooms, it possesses various health-promoting functions. In this study, the probiotic strains of Lactobacillus plantarum (L. plantarum) and Kluyeromyces marxianus (K. marxianus) were selected for cultivation and its effect on the growth and proliferation of PEP and PCP polysaccharide were examined. To understand the effects of different light sources irradiated mushroom polysaccharides for L. plantarum and K. marxianus’ growth. PEP was either unirradiated or light irradiated (gamma rays, pulse lights, LEDs and CCFL) and PCP was also either unirradiated or light irradiated (gamma and pulse lights). The thermal characteristics of the PEP and PCP were determined by differential scanning calorimetry (DSC). The findings indicated that more than a half onset temperature of light irradiated mushroom polysaccharides was decreased compared with the unirradiated polysaccharides. For the moisture desorption process and the scanning rate was 10 °C/min, the onset temperature for gamma irradiated PEP was decreased compared with the unirradiated sample. The onset temperatures for were 127.70, 128.97 and 129.48 °C for 3-10, 5-19 and 9-30 kGy, respectively. However, the onset temperature for the unirradiated PEP was 130.35 °C. The structure of the light irradiated mushroom polysaccharides was slightly changed compared with the unirradiated mushroom polysaccharides. It is believed that light irradiation may cause the structure of polysaccharides to become less compact and potentially damage their molecular construction. However, in this study, the observed changes were not significant and pronounced. In the TAM Air experiment, both unirradiated and light-irradiated mushroom polysaccharides proved to be suitable carbon sources in the media for L. plantarum. The growth curves for all conditions were satisfactory, indicating that L. plantarum can utilize energy from both PEP and PCP. The best performance was observed in the medium containing a mixture of mushroom polysaccharides and glucose. Interestingly, in the control group, the media containing 1% glucose outperformed those with 2% glucose, suggesting that excessively high sugar concentrations may hinder the growth of L. plantarum. Moreover, the growth rate of L. plantarum was relatively low in the medium containing only mushroom polysaccharides. Moreover, it was observed that neither PEP nor PCP served as suitable carbon sources for the growth of K. marxianus. The addition of mushroom polysaccharides did not improve the results in the medium for K. marxianus. Overall, PEP and PCP are suitable to replace the carbon sources partially or completely in the medium of L. plantarum. Besides, PEP and PCP are not suitable to replace the carbon sources in the medium for K. marxianu. It is proved that K. marxianus is more sensitive for the carbon source selection, and the concentration of glucose in the medium may affect K. marxianus growth directly.
    Appears in Collections:[食品營養與保健生技學系] 博碩士論文

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