廣藿香微體繁殖之研究

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Title:	廣藿香微體繁殖之研究
Other Titles:	Studies on Micropropagation of Pogostemon Cablin (Blanco) Benth.
Authors:	李敬慈 LI, CHING-TZU
Contributors:	闕甫伈 Chueh, FU-SHIN 食品營養與保健生技學系
Keywords:	植物組織培養;廣藿香;刺蕊草屬;唇形科;微體繁殖 plant tissue culture;Pogostemon cablin;Pogostemon;Lamiaceae;micropropagation
Date:	2023
Issue Date:	2023-11-22 01:12:47 (UTC+0)
Abstract:	唇形科植物廣泛分布於全球，包括許多重要的香草與藥用植物，其具有廣泛的生物活性，在預防和治療疾病方面具有重要的作用。2020年，新冠肺炎在全球迅速蔓延，有許多專家學者針對中草藥對於該疾病之預防及治療作用的研究，尤其進入後疫情時代，中醫藥的輔助治療，可以有效減輕癒後伴隨身體不適感。廣藿香( Pogostemon cablin )傳統是用來治療感冒、發燒、頭痛、腹瀉等效用，不僅可以用內服方式改善身體狀況，甚至還能利用芳香療法來達到淨化全身及環境，成為此疫情之下重要藥材之一。廣藿香屬於唇形科刺蕊草屬植物，主要含有萜類、黃酮類、醇、酸、酮、醛等多種化合物，其具有抗菌、抗氧化、抗癌、抗病毒及抗發炎等藥理作用，常運用於化妝品、食品或其他保健品等。本研究採用植物組織培養方法進行廣藿香之微體繁殖，改善栽培困境。研究結果顯示以莖節作為培植體可獲得最高誘導率，進一步將廣藿香不定芽繼代培養於含2 mg/L 6-benzylaminopurine (BA)以及0.2 mg/L 1-Naphthaleneacetic acid (NAA) 的 Murashige and Skoog (MS)基本鹽類培養基中除可獲得約6.6個芽體數外，亦可生長出較健全根系之植株，有助於馴化至野外。本研究建立廣藿香植物快速大量繁殖途徑，期盼能對國內廣藿香藥用植物的開發有所助益。 Lamiaceae plants are widely distributed worldwide, including many important herbs and medicinal plants. They exhibit diverse biological activities and play a significant role in preventing and treating diseases. In 2020, Coronavirus Disease-2019 (COVID-19) pandemic rapidly spread globally, prompting many experts and scholars to study the preventive and therapeutic effects of traditional Chinese herbal medicine, particularly in the post-pandemic era. Traditional Chinese medicine has been found to be effective in alleviating post-recovery discomfort. One such plant of interest is Pogostemon cablin. Traditionally, it has been used to treat conditions such as colds, fever, headaches, and diarrhea. Its medicinal properties can be harnessed through internal consumption or through aromatherapy to purify the body and the environment, making it an important medicinal herb during this pandemic. P. cablin belongs to the Lamiaceae family, in the genus Pogostemon. It contains various compounds, including terpenoids, flavonoids, alcohols, acids, ketones, and aldehydes. These compounds contribute to its pharmacological effects, such as antibacterial, antioxidant, anticancer, antiviral, and anti-inflammatory activities. P. cablin is commonly used in cosmetics, food, and other health products. To address cultivation challenges, this study utilized plant tissue culture techniques for the micropropagation of P. cablin. The researchers found that using stem nodes as explants yielded the highest induction rate. Further, by subculturing the P. cablin adventitious shoots on Murashige and Skoog (MS) basal salt medium supplemented with 2 mg/L of benzyladenine (BA) and 0.2 mg/L of naphthaleneacetic acid (NAA), they achieved approximately 6.6 shoot numbers and successfully developed healthier root systems in the plants, making them more adaptable for cultivation in the wild. This study established a rapid and efficient way to mass propagate P. cablin and is expected to contribute to the development of this medicinal plant in the country.
Appears in Collections:	[Department of Food Nutrition and Healthy Biotechnology] Theses & dissertations

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