Tridax procumbens Linn. is used in folk medicine. It is reported that the plant shows antibacterial activity and contains antitumor chemical components. The purpose of this research was to evaluate antibacterial, antioxidation, anti-inflammation and anticancer activity of T. procumbens Linn. The flowers , leaves and stems of T. procumbens were extracted with ten times of ethyl alcohol. The extract was then partitioned by n-hexane, ethyl acetate, n-butyl alcohol and water to evaluate its antimicrobial and antioxidative activities. We also evaluate its anti-inflammation and anticancer activity of the flower. The result showed that most of extracts had antimicrobial activities extracts from flower. The ethyl acetate extract was the most effective among all the extracts. Most extracts from flowers, leaves and stems showed the good antioxidative activities especially the flower. The the ethyl acetate extracts presented the best on DPPH scavenging activity and reducing power and had the highest total phenolic compounds content . We used the ethyl alcohol and water extracts of T. procumbens to treat RAW264.7 macrophage, which induced inflammation by Lipopolysaccharide (LPS).In the NO assay, the extracts of T. procumbens flower had better inhibit In MTT assay of SK-Hep-1, we found that extract of T. procumbens flower had the best inhibitory ability. We also found the ethyl alcohol extract of T. procumbens had the best to diminish the expression of matrix metalloproteinase (MMP)-9 and MMP-2. The ethyl alcohol extracts of flower had good anti-migration and anti-invasion ability.
