| Abstract: | Diabetes was one of the major chronic diseases in Taiwan. Hyperglycemia-induced oxidative stress may play an important role in the development of chronic complications in diabetes mellitus. Guava (Psidium guajava L.) is a tropical fruit, which belongs to the family Myrtaceae, it is one of the most important economic fruit in Taiwan. Leaves and fruits of guava have been reported to have anti-diarrheal, hypoglycemic, lipid lowering, anti-bacterial and anti-oxidative activities. This study was divided into two parts, first part is to evaluate the ethanol and ethyl acetate extract of guava which anti-oxidative and anti-inflammatory ability. The second part of study is to observe the effects of guava supplementation in STZ-induced type 2 diabetes mice. All Balb/c mice were classified into seven groups as follows: normal group (N), diabetes group (DM), guava supplement groups (L, M, H), 5% guava and rosiglitazone supplement group (HR), and rosiglitazone group (R). In vitro study showed that the ethanol extract of guava has better anti-oxidative and anti-inflammatory activities. In vivo study showed that guava supplement groups could significantly improved the blood sugar, insulin resistance, creatinine, blood urea nitrogen, trigylcerides, cholesterol, non-esterified free fatty acids, C-reactive protein than diabetes group. As a result of PPAR??activation lead to reduce the iNOS and NF-?B protein expression and increase the GPx3 and ACO protein expression. These results indicated that guava supplement could attenuated the diabetic condition and possessed the pontential to developed the healthy foods. |
