The aim of this research was used the waste milkfish scale to extract collagen and develop facial mask product. The collagen derived from milkfish scales were produced by different pretreatment and extracting methods. Basing on the test of L.a.b value, ash content, calcium content, solid content, hydroxyproline content, and sensory evaluation, it was found that blanching, decalcification, extracting with pepsin, and deordorization are the four key points to improve the fish scale collagen yield and quality. In this study the optimal collagen-extracting ratio and solid recovery were 73.27% and 17.37% in respectively. It showed that 5-30kDa molecular weight of collagen has the better scavenging activity of DPPH radical、 reducing power and chelation activity on ferrous ion. When collagen facial mask liquid contains 15% polyvinyl alcohol, it can be observed, has the highest liquid viscosity and film forming ability. The liquid collagen mask have the excellent ability to maintain the moisture of skin, and they were very stable without phase separation during 60 day stability test periods.
