ASIA unversity:Item 310904400/11481
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    题名: The effects of growth regulators and sucrose on the production of proanthocyanidin ellagic acid and resveratrol in Vitis vinifera cell cultures
    作者: Ming-Chien, Hsu
    贡献者: Department of Biotechnology
    关键词: grape;cell cultures;secondary metabolites
    日期: 2011
    上传时间: 2011-09-26 02:38:03 (UTC+0)
    出版者: Asia University
    摘要: The antioxidants in grapes such as proanthocyanidins, ellagic acid and resveratrol are usually applied in food, medical and cosmetic products. Previous studies have shown that the changes of environmental factors such as UV light treatment, sucrose concentration and plant growth regulators, could increase the contents of these antioxidants in Vitis vinifera L. Gamay Freaux cultures. Therefore, we use 54 kinds of suspension culture conditions which use plant growth regulators for the FC-01 grape callus (Vitis vinifera L. Gamay Freaux). The extracts(proanthocyanidins, ellagic acid and resveratrol) from FC-01 were tested with inhibition of tyrosinase activity. The results have shown FC-01 in suspension culture conditions for the 0.3 mg/L NAA + 0.3 mg/L kinetin + 50g/L sucrose cultured in the amount of proanthocyanidin biosynthesis up to 7.541 mg/L. By applying the suspension culture condition (0.1 mg/L NAA + 0.1 mg/L kinetin + 30 g/L sucrose), we were able to induce proanthocyanidins, ellagic acid and resveratrol biosynthesis. Additionally, by applying another suspension culture condition (0.1 mg/L 2, 4-D + 0.2 mg/L kinetin + 30 g/L sucrose), we were also able to culture the amount of resveratrol up to 5.212 mg/L. The tyrosinase activity inhibition test showed that the amount of biosynthesis of proanthocyanidins, ellagic acid and resveratrol was not significantly related. Furthermore, this experiment has shown that different plant growth factors such as the regulation of sucrose concentration and plant hormone concentration can induce an increase in the amount of the biosynthesis of proanthocyanidins, ellagic acid and resveratrol in FC-01, and subsequently improve the value of the use of FC-01.
    显示于类别:[生物科技學系] 博碩士論文

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