Molecular characterization of the M and L RNAs of Tomato yellow ring virus and the M RNA of Peanut chlorotic fan-spot virus

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 博碩士論文 > Item 310904400/11480

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題名:	Molecular characterization of the M and L RNAs of Tomato yellow ring virus and the M RNA of Peanut chlorotic fan-spot virus
作者:	Ya-Hsu Fan
貢獻者:	Department of Biotechnology
關鍵詞:	Tospovirus;Tomato yellow ring virus, TYRV;Peanut chlorotic fan-spot virus, PCFV
日期:	2011
上傳時間:	2011-09-26 02:38:02 (UTC+0)
出版者:	Asia University
摘要:	Tospovirus, the only plant-infecting genus in the family Bunyaviridae, has enveloped virions of 80-110 nm in diameter consisting of three segmented single-stranded RNA genome, denoted S, M and L, for coding six viral proteins. Both S and M RNAs are ambisense and L RNA has a negative polarity. A nonstructural NSs protein, acting as gene-silencing suppressor, and the RNA-binding nucleocapsid protein (NP) are encoded from the S RNA. The M RNA is responsible for coding a nonstructural NSm protein involved in cell-to-cell movement and the precursor of two glycoproteins on the surface of viral envelope. The L RNA codes the RNA-dependent RNA polymerase (RdRp) for replication and transcription. Tomato yellow ring virus (TYRV), isolated from tomato in Iran, and Peanut chlorotic fan-spot virus (PCFV), from peanut in Taiwan, were classified as two tentative species of the genus Tospovirus. To clarify the taxonomic status of these two viruses, the M and L RNAs of TYRV and the M RNA of PCFV were determined and analyzed in this investigation. The M RNA of TYRV has 4786 nucleotides (nt) coding for a NSm protein of 308 amino acids (aa) (34.5 kDa) and a glycoprotein precursor (GP) of 1310 aa (128 kDa). The L RNA of TYRV has 8877 nt encoding an RdRp of 2873 aa (331 kDa). The M RNA of PCFV has 4786 nt encoding a NSm protein of 306 aa (34.3 kDa) and a GP of 1111 aa (126.3 kDa). The NSm and GP proteins of TYRV share high 89.9% and 80.1-86.5% aa identities, respectively, with those of Polygonum ringspot virus (PolRSV) and Iris yellow spot virus (IYSV). However, the NSm and GP proteins of PCFV share low identities of 34.6-42.7% and 31.1-33.3%, respectively, with those of other tospovirus species. The RdRp of TYRV shares the highest aa identity (88.7%) with that of IYSV. Sequence analyses indicate that TYRV and PCFV should be classified as official species of the genus Tospovirus. TYRV is closely related to IYSV and PolRSV, but PCFV is distant from other tospoviruses.
顯示於類別:	[生物科技學系] 博碩士論文

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