Echinacoside promotes protective effect against photoaging on Human dermal fibroblast cells following UVB exposure

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Title:	Echinacoside promotes protective effect against photoaging on Human dermal fibroblast cells following UVB exposure
Authors:	Noriega, Lloyd Noriega, Lloyd
Contributors:	生物科技學系
Keywords:	Photoaging;Aging;Echinacoside;UVB;IG1G1R;Collagen
Date:	2020
Issue Date:	2022-12-14 06:46:15 (UTC+0)
Publisher:	亞洲大學
Abstract:	Part 1: Echinacoside inhibits UVB-induced Photoaging by activating the IGF1R signaling pathway in HS68 human dermal fibroblastAbstract Impaired collagen synthesis caused by aging results in the changes in skin appearance, such as wrinkle formation. Accordingly, reviving the amount of collagen may prevent skin from senescence. Ultraviolet (UV) irradiation is the most leading factor contributing to skin photoaging, including reactive oxygen species (ROS) formation, collagen breakdown and skin damage. Echinacoside (Ech), a natural phenylethanoid glycoside, is the most abundant active compound found in Herba Cistanches. Previous studies showed that Ech possesses antioxidant and anti-apoptotic properties. However, whether it can benefit the skin and the relevant molecular mechanism or not is still unclear. The objective of this research is to investigate the effect of Ech in Hs68 human dermal fibroblast cells under UVB-induced photo-aging. In our results, it showed that Ech could increase the level of collagen in Hs68 cells through IGF1/IGF-1R/ERK collagen synthesis pathway. Aging-associated marker such as p21, p53 and β-gal decreased following Ech treatment under UVB exposure, which indicates its anti-aging effect. Moreover, increased cellular senescence seems crucial in Ech-mediated promoting IGF1/IGF-1R/ERK collagen synthesis pathway. This was also evidenced from IGF-1R knocked-out gene in which Echinacoside was not able to suppress the UVB-induced senescence generation in the absence of IGF-1R. In our study, we provide the evidence that Ech can be used to treat immature aging caused by UV and be a potential candidate for developing a natural anti-aging product to protect the skin from UVB-induced damage.Part 2 : Echinacoside promotes Antioxidant Activity in human dermal fibroblast cells via activation of Nrf2/ARE PathwayAbstract The aim of this research was to examine the antioxidant property of the Ech, which is the most abundant compound found in herba cistanche. Ech is a natural polyphenol which have a Neuroprotection, antioxidant, anti-inflammation and anti-diabetic properties. The antioxidant mechanism in skin is still unexplored. We investigated intracellularly the antioxidant properties of Ech and the molecular mechanism involved in HS68 normal human fibroblast cells. The result showed that Ech dose-dependently increased the original form and phosphorylated form of Nrf2 which is the ‘’master regulator” of antioxidant. We also observed the increase of the downstream gene of Nrf2 such as SOD1, γGCSc, Catalase and Ho-1. By doing time dependent experiment we demonstrated the translocation of Nrf2 into nucleus. Furthermore, silencing of Nrf2 (siRNA transfection) diminished the Ech-mediated increase antioxidant effect, as evidenced by impaired antioxidant genes, uncontrolled ROS and cellular senescence production, following the UVB irradiation. Our result revealed that Ech could increase antioxidant proteins through the Nrf2/ARE signaling pathway by translocating to nucleus and binding to ARE promoter region to mediate the increase of antioxidant gene. We found that Echinacoside can be a natural compound to potentially protect our skin from oxidative stress caused by ROS production in Human Dermal Fibroblast cell.
Appears in Collections:	[Department of Biotechnology] Other Research Project

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