近年來，由於食安問題層出不窮，國人們對於食品相關議題也越來越重視，肉品中的萊克多巴胺 (Ractopamine , RAC) 與食品病原菌單核細胞增多性李斯特菌 (Listeria monocytogenes) 皆有可能造成人體危害與食品安全風險。目前上述危害之傳統公告檢測方法耗時費力或需高階儀器，因此對於尋求可快速、靈敏、簡單的篩檢方法有所需求，近年來，電化學阻抗圖譜 (Electrochemical impedance spectroscopy, EIS) 技術與生物晶片具上述之優點，因此本研究乃利用EIS技術結合新型生物晶片進行RAC與L. monocytogenes檢測方法之開發。本論文結果發現，EIS結和生物晶片可降低實驗時間，且晶片之靈敏度高，可檢測到較低濃度的RAC或L. monocytogenes。結果顯示本研究以利用半導體生物傳感器檢測RAC檢測濃度可至0.01ppb；而利用金傳感器檢測L. monocytogenes檢測濃度可至101 CFU/mL。 In recent years, as food safety problems have emerged, people are paying more and more attention to food-related issues. Ractopamine in meat and food pathogen Listeria monocytogenes may both cause human health problems, hazards, and food safety risks.At present, the traditional detection methods of the above-mentioned hazards are time-consuming and labor-intensive or require high-end instruments. Therefore, there is a need for a fast, sensitive and simple screening method. In recent years, Electrochemical impedance spectroscopy (EIS) technology and biosensors have the above advantages, so this study is to use EIS technology combined with new biosensors to develop the detection method of Ractopamine and L. monocytogenes.The results of this thesis found that food safety biosensors can reduce the experimental time, and the chip has high sensitivity, which can detect lower concentrations of Ractopamine or L. monocytogenes.The results show that the concentration of Ractopamine detected by the semiconductor biosensor can reach 0.01 ppb, and the concentration of L. monocytogenes can be detected by the gold sensor can reach 101 CFU/mL.
