1,25(OH)2 D3 attenuates indoxyl sulfate-induced epithelialto-mesenchymal cell transition via inactivation of PI3K/Akt/b-catenin signaling in renal tubular epithelial cells

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题名:	1,25(OH)2 D3 attenuates indoxyl sulfate-induced epithelialto-mesenchymal cell transition via inactivation of PI3K/Akt/b-catenin signaling in renal tubular epithelial cells
作者:	Cha, Li-Chien Chang, Li-Chien Sun, Hai-Lun Sun, Hai-Lun Tsa, Chia-Han Tsai, Chia-Han Kuo, Chia-Wen Kuo, Chia-Wen Liu, Kai-Li Liu, Kai-Li 李宗貴 Lii, Chong-Kuei 黃晉修 Huang, Chin-Shiu Chien-Chun, L Li, Chien-Chun
贡献者:	醫學暨健康學院食品營養與保健生技學系
关键词:	1,25(OH)2 D3;β-catenin;EMT;HK-2 cells;Indoxyl sulfate
日期:	2020-01-20
上传时间:	2020-10-14 05:43:12 (UTC+0)
出版者:	亞洲大學
摘要:	Objectives Indoxyl sulfate (IS), a uremic toxin, has been shown to promote the epithelial-to-mesenchymal transition (EMT) of human proximal tubular cells and to accelerate the progression of chronic kidney disease (CKD). Despite the well-known protective role of 1,25-dihydroxyvitamin D3 [1,25(OH)2 D3] in EMT, the effect of 1,25(OH)2 D3 on IS-induced EMT in human proximal tubular epithelial cells and the underlying mechanism remain unclear. The aim of this study was to determine whether IS (0-1 mM) dose-dependently inhibited the protein expression of E-cadherin and increased the protein expression of alpha-smooth muscle actin, N-cadherin, and fibronectin. Methods This study investigated the molecular mechanism by which 1,25(OH)2 D3 attenuates IS-induced EMT. HK-2 human renal tubular epithelial cells was used as the study model, and the MTT assay, Western Blotting, siRNA knockdown technique were used to explore the effects of 1,25(OH)2 D3 on EMT in the presence of IS. Results Pretreatment with 1,25(OH)2 D3 inhibited the IS-induced EMT-associated protein expression in HK-2 cells. IS induced the phosphorylation of Akt (S473) and β-catenin (S552) and subsequently increased the nuclear accumulation of β-catenin. Pretreatment with 1,25(OH)2 D3 and LY294002 (phosphoinositide 3-kinase [PIK3] inhibitor) significantly inhibited the IS-induced phosphorylation of Akt and β-catenin, nuclear β-catenin accumulation, and EMT-associated protein expression. Conclusions Results from the present study revealed that the anti-EMT effect of 1,25(OH)2 D3 is likely through inhibition of the PI3K/Akt/β-catenin pathway, which leads to down-regulation of IS-driven EMT-associated protein expression in HK-2 human renal tubular epithelial cells.
關聯:	NUTRITION
显示于类别:	[食品營養與保健生技學系] 期刊論文

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