Gefitinib and curcumin-loaded nanoparticles enhance cell apoptosis in human oral cancer SAS cells in vitro and inhibit SAS cell xenografted tumor in vivo.

ASIAIR > College of Medical and Health Science > Department of Food Nutrition and Healthy Biotechnology > Journal Article > Item 310904400/112884

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Title:	Gefitinib and curcumin-loaded nanoparticles enhance cell apoptosis in human oral cancer SAS cells in vitro and inhibit SAS cell xenografted tumor in vivo.
Authors:	KC, Lai;KC, Lai;闕甫伈;FS, Chueh;YT, Hsiao;YT, Hsiao;ZY, Cheng;ZY, Cheng;JC, Lien;JC, Lien;KC, Liu;KC, Liu;SF, Peng;SF, Peng;JG, Chung;JG, Chung
Contributors:	食品營養與保健生技學系
Keywords:	Gefitinib;Curcumin;γ-PGA-Cur/Gef nanoparticles;SAS human oral cancer cells;Apoptosis
Date:	2019-11
Issue Date:	2020-09-01 07:11:01 (UTC+0)
Publisher:	亞洲大學
Abstract:	Curcumin (Cur), a natural product, has been shown to have anti-tumor activities in many human cancer cells. Gefitinib (Gef) is a clinical drug for cancer patients. However, there is no available information to show whether Gef/Cur nanoparticles (NPs) increased cell apoptosis and anti-tumor effects on xenograft mice model in vivo. In this study, γ-polyglutamic acid-coated nanoparticles loaded with Gef and Cur (γ-PGA-Gef/Cur NPs) were developed and its physicochemical properties and antitumor effects were investigated in vitro and in vivo. The γ-PGA-Gef/Cur NPs showed 548.5 ± 93.7 nm in diameter and −40.3 ± 3.87 mV on surface charge. The loading efficiencies of Gef and Cur were 89.5 and 100%, respectively. γ-PGA-Gef/Cur NPs could be internalized into SAS cells and significantly decreased total cell viability of SAS cells. Western blotting results indicated that both free Gef/Cur and γ-PGA-Gef/Cur NPs induced apoptotic cell death via caspase- and mitochondria-dependent pathways. In vivo studies indicated that treatments of PLGA NPs, free Gef/Cur, and γ-PGA-Gef/Cur NPs did not significantly affect appearances and bodyweights of mice. But the γ-PGA-Gef/Cur NPs significantly suppressed tumor size when comparing to free Gef/Cur-treated group. The nanoparticles developed in this study may be used as a potential therapy for oral cancer.
Relation:	TOXICOLOGY AND APPLIED PHARMACOLOGY
Appears in Collections:	[Department of Food Nutrition and Healthy Biotechnology] Journal Article

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