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| 題名: | Pulmonary fibroblasts-secreted CXCL10 polarizes alveolar macrophages under pro-inflammatory stimuli |
| 作者: | 蔡政芳;Tsai, Cheng-Fang;Che, Jia-Hong;Chen, Jia-Hong;Yeh, Wei-Lan;Yeh, Wei-Lan |
| 貢獻者: | 醫學檢驗暨生物技術學系 |
| 關鍵詞: | Alveolar Macrophage；CXCL10；Inflammation；Polarization；Pulmonary Fibroblast |
| 日期: | 2019-10 |
| 上傳時間: | 2020-08-28 07:00:21 (UTC+0) |
| 出版者: | 亞洲大學 |
| 摘要: | Background
During acute lung injury, lung fibroblasts produce chemokines that assist the activation and migration of resident macrophages. The interactions between pulmonary fibroblasts and alveolar macrophages demonstrate the early event in the recruitment of immune cells, and the production of chemokines appear to be central mediators of the initiation and progression of inflammatory responses. In this study, the aim was to investigate the signaling pathway leading to CXCL10 secretion and the effects of CXCL10 released by activated fibroblasts on regulating macrophage polarization in a pro-inflammatory microenvironment.
Methods
The expression of chemokines CCL2, CCL5, CXCL10, and CXCL12, and the phosphorylation of signaling molecules STAT3, FAK, GSK3αβ and PKCδ were investigated by real time-PCR, ELISA, or Western blot on TNFα- or IL-1β-activated MRC-5 pulmonary fibroblasts. By collecting conditioned medium from TNFα-activated fibroblasts, the expression of iNOS and arginase I on MH-S alveolar macrophages were examined by real-time PCR. Surface markers CD86 and CD206 expressions on alveolar macrophages were also evaluated by flow cytometry.
Results
We found that CXCL10 production was significantly elevated on MRC-5 fibroblasts under TNFα- or IL-1β treatment. In addition, we revealed that TNFα and IL-1β initiated phosphorylation of STAT3, FAK, GSK3αβ and PKCδ signaling cascade, leading to the elevation of CXCL10 expression. Moreover, conditioned medium collected from TNFα-activated MRC-5 fibroblasts increased iNOS and CD86 expressions and decreased arginase I and CD206 expressions on MH-S alveolar macrophages, and neutralization of CXCL10 abolished these observed phenomena.
Conclusion
These results suggest that CXCL10 is crucial in activated fibroblasts-promoted M1 phenotype polarization of alveolar macrophages. In this regard, targeting fibroblasts-released CXCL10 may be promising as anti-inflammatory therapy against acute lung injury. |
| 關聯: | TOXICOLOGY AND APPLIED PHARMACOLOGY |
| 顯示於類別: | [醫學檢驗暨生物技術學系] 期刊論文
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