Combined Therapy With Hyperbaric Oxygen and Melatonin Effectively Reduce Brain Infarct Volume and Preserve Neurological Function After Acute Ischemic Infarct in Rat.

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Title:	Combined Therapy With Hyperbaric Oxygen and Melatonin Effectively Reduce Brain Infarct Volume and Preserve Neurological Function After Acute Ischemic Infarct in Rat.
Authors:	林坤成;Lin, Kun-Chen;陳冠宏;Chen, Kuan-Hung;Christopher;Wallace, Christopher Glenn;陳怡伶;Chen, Yi-Ling;高常發;Ko, Sheung-Fat;李炫昇;Lee, Mel S;葉漢根;Yip, Hon-Kan
Contributors:	護理學系
Keywords:	Acute ischemic stroke;Angiogenesis;Hyperbaric oxygen;Inflammation;Melatonin;Neurological function;Oxidative stress.
Date:	2019-10
Issue Date:	2020-08-28 06:54:29 (UTC+0)
Publisher:	亞洲大學
Abstract:	This study tested the hypothesis that combined hyperbaric oxygen (HBO) and melatonin (Mel) was superior to either one for protecting the brain functional and parenchymal integrity from acute ischemic stroke (IS) injury. Adult-male Sprague-Dawley rats were divided into groups 1 (sham-operated control), 2 (IS), 3 (IS + HBO), 4 (IS + Mel), and 5 (IS + HBO-Mel). By day 28 after IS, the brain infarct area (BIA) was lowest in group 1, highest in group 2, significantly higher in groups 3 and 4 than in group 5, but not different between groups 3 and 4. The neurological function at day 7, 14, and 28 exhibited an opposite pattern to BIA among the 5 groups. The protein expressions of inflammatory (IL-1β/IL-6/iNOS/TNF-α/p-NF-κB), apoptotic (cleaved-caspase3/cleaved-PARP/mitochondrial Bax), mitochondrial/DNA-damaged (cytochrome-C/γ-H2AX), oxidative stress (NOX-1/NOX-2), and autophagy (i.e. ratio of CL3B-II/CL3B-I) biomarkers displayed an identical pattern of BIA among 5 groups. Cellular expressions of inflammation (F4/80+/GFAP+) and DNA-damaged biomarker (γ-H2AX+) exhibited an identical pattern, whereas the integrities of myelin sheath/neuron (MPB+/NeuN+), endothelial cell (CD31+/vWF+), and number of small vessels exhibited an opposite pattern of BIA among the 5 groups. Combined HBO-Mel therapy offered an additional benefit in protecting the brain against IS injury.
Relation:	JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
Appears in Collections:	[護理學系] 期刊論文

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