Direct implantations of erythropoietin and autologous EPCs in critical limb ischemia (CLI) area restored CLI area blood flow and rescued remote AMI-induced LV dysfunction.

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题名:	Direct implantations of erythropoietin and autologous EPCs in critical limb ischemia (CLI) area restored CLI area blood flow and rescued remote AMI-induced LV dysfunction.
作者:	李芳艷;Lee, Fan-Yen;羅啟文;Luo, Chi-Wen;Christopher;Wallace, Christopher Glenn;陳冠宏;Chen, Kuan-Hung;許俊傑;Sheu, Jiunn-Jye;鄞宗誠;Yin, Tsung-Cheng;柴漢東;Chai, Han-Tan;葉漢根;Yip, Hon-Kan
贡献者:	護理學系
关键词:	Primary and remote organ damageErythropoietinEndothelial progenitor cellAngiogenesis
日期:	2019-10
上传时间:	2020-08-28 06:52:55 (UTC+0)
出版者:	亞洲大學
摘要:	Background This study tested the hypothesis that intramuscular injections of erythropoietin (EPO) and endothelial progenitor cells (EPC) to critical limb ischemia (CLI; primary treatment site) could also improve heart function in rat after acute myocardial infarction (AMI; remote ischemic organ). Method Adult-male SD rats (n = 40) were equally categorized into group 1 (sham-operated control), group 2 (CLI-AMI), group 3 [CLI-AMI + EPO (10 mg/kg)], group 4 [CLI-AMI + EPCs (1.2 × 106)] and group 5 (CLI-AMI + EPCs + EPO). Results By day 21 (end of study period), 2-D echo and Laser doppler showed that left-ventricular injection fraction (LVEF) and the ratio of ischemic to normal blood flow were highest in group 1, lowest in group 2, significantly higher in group 5 than in groups 3 and 4, but not different in the latter two groups (all p < 0.0001). Flow cytometry and ELISA demonstrated that circulating angiogenesis factors were significantly progressively increased from groups 1 to 5 (all p < 0.001). The number of small vessels and protein (CD31/eNOS)/cellular (vWF) expressions reflecting integrity of endothelium exhibited an identical pattern to LVEF whereas protein (VEGF/SDF-1α)/cellular (VEGF) expressions were significantly progressively increased from groups 1 to 5 in quadriceps and heart tissues (all p < 0.0001). Protein expressions of apoptotic (Bax/caspase-3/PARP)/inflammatory (MMP-9) and microscopic findings of ischemic/fibrotic/collagen-deposition areas and DNA-damage marker (γ-H2AX+) were lowest in group 1 and significantly progressively decreased from groups 2 to 5 in quadriceps and heart tissues (all p < 0.0001). Conclusions Direct injection of EPO-EPC into CLI effectively restored blood flow in the CLI area and also preserved remote heart function.
關聯:	BIOMEDICINE & PHARMACOTHERAPY
显示于类别:	[Department of Nursing] Journal Article

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