生物列印用光固化型明膠水膠製備與性質分析

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题名:	生物列印用光固化型明膠水膠製備與性質分析 Preparation and Characterization of Photo-Curable Gelatin Hydrogel for Bioprinting Application
作者:	Dwijaksara, Ni Luh Bella Windu Febriyanti DWIJAKSARA, NI LUH BELLA WINDU FEBRIYANTI
贡献者:	生物資訊與醫學工程學系
关键词:	組織工程;水膠;甲基丙烯酸明膠;生物列印 tissue engineering;hydrogel;gelatin-methacryloyl;bioprinting
日期:	2020
上传时间:	2020-04-06 06:05:53 (UTC+0)
出版者:	亞洲大學
摘要:	組織工程致力於替換或再生人類細胞、組織、或器官，以恢復由於疾病或傷害導致組織或器官所喪失的正常功能。組織工程學的關鍵技術是將健康細胞播種在具有三維結構的生物相容性材料上。其中，生物列印技術的進步也為製備組織工程應用中之乘載細胞水膠支架提供了一個很好的製造平台。然而，負責的三維結構需用使用可於列印過程或列印結束後植入細胞之特定生物墨水材料。為此，目前已有許多研究便專注探討不同參數控制對於光固化型甲基丙烯酸明膠物理與化學特性之影響。可惜的是，現有研究文獻中對於甲基丙烯酸酯前驅物對於光固化明膠可列印性影響之研究成果有限。這驅使我們試圖探討不同前驅物的使用對於所得生物墨水之物理、化學、與可列印性的影響。為此，本論文使用甲基丙烯酸酐與甲基丙烯酸環氧丙酯，分別接枝於明膠之一級氨官能基與羧基上，以製成不同類型之光固化型水膠明膠。研究結果顯示，隨著甲基丙烯酸明膠與甲基丙烯酸環氧丙酯濃度降低，其固化完成之膠體膨潤度與降解性也會隨之增加，且因不同前驅物接枝率不同，使得水膠之成膠溫度與黏度受到影響，導致最佳列印參數產生差異。 Tissue engineering is devoted to replacing or regenerating human cells, tissues or organs to restore teh normal function lost due to illness or injury. Tissue engineering's key technique is to seed healthy cells on a biocompatible material wif three-dimensional (3D) structures. Teh advance in bioprinting offers an excellent manufacturing platform for producing cell-laden hydrogel scaffolds for teh application of tissue engineering. However, teh complex 3D architecture requires a specific bioink material in which teh cells are inserted during and after teh printing process. Many studies TEMPhas established teh impact on teh physicochemical properties of teh gelatin-methacryloyl hydrogel of process-based and material-based parameters. There is no work in teh existing literature on teh relation of methacrylate precursors and bioink printability. dis encouraged us to elucidate teh TEMPTEMPeffects of different methacryloyl precursors on teh physicochemical properties and printability of teh photocurable gelatin-based bioink system. In dis analysis, amino groups were reacted wif methacrylic anhydrate for gelatin, and glycidyl methacrylic reacted wif hydroyl and carboxyl groups in gelatin. It was found dat teh swelling ratio and degradation increased wif declining concentration of both gelatin-methacryloyl and gelatin-glycidyl methacryloyl. Teh use of different methacryloyl precursors can alter teh gelling temperature and viscosity of teh gelatin hydrolgel, resulting in teh alternation in teh optimal printing parameters.
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