癌症轉錄組數據中ECI2異構體的定量分析

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Title:	癌症轉錄組數據中ECI2異構體的定量分析 Quantitative Analysis of ECI2 Isoforms from Cancer Transcriptomic Data
Authors:	顏名儀 YEN, MING-YI
Contributors:	生物資訊與醫學工程學系
Keywords:	ECI2;癌症;轉錄組分析;MammaPrint ECI2;Cancer;Transcriptome analysis;MammaPrint
Date:	2019
Issue Date:	2019-10-22 01:23:32 (UTC+0)
Publisher:	亞洲大學
Abstract:	轉錄組的量化分析近年來逐漸受到重視，也愈來愈多研究指出轉錄組異構體在疾病上可能具有關鍵的致癌機制。本研究發現ECI2 (Homo sapiens enoyl-CoA delta isomerase 2 )為哺乳動物的一種過氧化物酶體的異構酶，共具有11條轉錄組異構體，其中一條ECI2異構體被發現為重要的癌抗原，稱為肝細胞癌相關抗原64（HCA64）。在其他研究上，HCA64還被發現可作為其他癌症如乳腺癌和前列腺癌的預後的基因標記。　　RNA-Seq高通量測序已經成為測量基因表現量的最先進方法之一。因此本文使用Salmon軟體並利用RNA-seq資料進行轉錄物表現量分析，並針對乳腺癌、小細胞肺癌、卵巢癌、結直腸癌及前列腺癌五種癌正進行分析，期望可以找出HCA64與特定癌症的關係，以作為該癌症的生物標誌物。如此將有益於癌症診斷及後續治療，並提高癌症病人的存活率。　　根據研究結果顯示HCA64的轉錄物僅在部分癌症樣品中表現，經過DESeq2分析，分別在轉移性乳癌、前列腺癌及肝癌上找出具有顯著性的基因。此些有顯著性差異的基因，有許多都在上述三種癌症中具有作用機制；因此，可推論HCA64於轉移性乳癌、前列腺癌及肝癌間具有重要的關聯。 Quantitative analysis of transcriptomes has received increasing attention in recent years, and more and more studies have confirmed that transcriptome isomers may have a key mechanism in cancers. Recently, the study found that ECI2 (Homo sapiens enoyl-CoA delta isomerase 2) is a peroxisome isomerase of mammals with 11 transcriptome isoforms, one of which is found to be an important cancer antigen, called Hepatocellular Carcinoma Antigen 64 (HCA64). In other studies, it has also been found as a biomarker for the prognosis of other cancers such as breast cancer and prostate cancer. RNA-Seq high-throughput sequencing has become one of the most advanced methods for measuring gene expression. Therefore, we use of RNA-seq data to analyze the transcript expression of the software Salmon and to analyze the expression of the transcriptome for breast cancer, small cell lung cancer, ovarian cancer and prostate cancer. It is expected that the relationship between HCA64 and a specific cancer can be found, which may serve as a biomarker for the cancer, which is useful for diagnosis and subsequent treatment, and improves the survival rate of the patient. According to the results of the study, the transcript of HCA64 was only expressed in some cancer samples. After DESeq2 analysis, significant genes were found in metastatic breast cancer, prostate cancer and liver cancer, and many of these genes have a related mechanism in developing three cancers. It can be inferred that HCA64 has an important association in metastatic breast cancer, prostate cancer and liver cancer.
Appears in Collections:	[生物資訊與醫學工程學系 ] 博碩士論文

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