Inhibitory effect of berberine on interleukin-2 secretion from PHA-treated lymphocytic Jurkat cells

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Title:	Inhibitory effect of berberine on interleukin-2 secretion from PHA-treated lymphocytic Jurkat cells
Authors:	Hu, Sindy Hu, Sindy Ch, Chien-Wei Chen, Chien-Wei Chen, Szu-Tah Tsui, Ke-Hung Tan, Twen-Kei Tang, Twen-Kei Che, Hao-Tsai Cheng, Hao-Tsai Guey-Shyang Hwang, Guey-Shyang Yu, Ju-Wen Li, Yi-Chieh 王錫崗 Wang, Paulus S.
Contributors:	生物科技學系
Date:	2018-12
Issue Date:	2019-09-10 05:17:47 (UTC+0)
Abstract:	Berberine is an isoquinoline alkaloid isolated from herb plants, such as Cortex phellodendri (Huangbai) and Rhizoma coptidis (Huanglian). Huanglian and Huangbai have been used as “heat-removing” agents. In addition, berberine has been reported to exert anti-inflammatory effect both in vivo and in vitro, where mitogen-activated protein kinase (MAPK) and cyclooxygenase-2 (COX-2) expressions are critically implicated. We herein tested the hypothesis that berberine exerts an anti-inflammatory effect through MAPK and COX-2 signaling pathway in T-cell acute lymphoblastic leukemia (T-ALL). In Jurkat cells, we found that PHA exposure caused elevation on interleukin-2 (IL-2) production in a time-dependent manner. PHA-stimulated reactions were steeply suppressed by berberine, such as IL-2 mRNA expression and protein secretion. However, berberine did not exert any cytotoxic effect at doses of 40 μg/ml. In addition, the possible molecular mechanism of anti-inflammation effect of berberine could be the inhibition of PHA-evoked phosphorylation of p38, since c-Jun N-terminal kinases (JNK) and extracellular signal-regulated kinase (ERK) expressions did not alter. Consistent with above results, berberine inhibition on PHA-induced IL-2 secretion could be reversed by treatment of SB203580, a specific inhibitor of p38-MAPK. Interestingly, upregulation of PHA-induced COX-2 expression was also observed following berberine treatment of Jurkat cells. Furthermore, flow cytometry analysis showed berberine-induced cell cycle arrest at G1 phase after PHA stimulation and decreased percentage of G2/M phase. In conclusion, our study demonstrated that the anti-inflammatory effect of berberine largely potentially results from its ability to attenuate p38 MAPK expression, and does not exclude a positive action of berberine on cell cycle arrest. These results provide an innovative medicine strategy to against or treat T-ALL patients.
Relation:	INTERNATIONAL IMMUNOPHARMACOLOGY
Appears in Collections:	[Department of Biotechnology] Journal Article

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